Expression And Clinical Significance Of MiR-150 And MiR-155 In The Serum From Patients With Diabetic Nephropathy

Aims:To investigate the expression and clinical significance of serum miRNA expression profiling in the occurrence and progression of diabetic nephropathyMethods:The miRNA expression profiling was detected by miRNA TaqMan Low Density Array chip from 10 patient with diabetic nephropathy,10 diabetes patients with normoalbuminuria and 10 health control. Real-time quantitative PCR(RT-PCR) was applied to verify the result of miRNA array in serum samples of 66 patients with diabetic nephropathy (36 patients with microalbuminuria,30 patients with macroalbuminuria),40 diabetes patients with normoalbuminuria and 40 health control.And the relationship of differetial expression with clinical features was analyzed.Results:miR-155-5p, miR-150, miR-30e, miR-3196, miR-320e and let-7a-5p being validated by RT-PCR differentially expresse in 3 groups of serum samples from the diabetes patients with microalbuminuria (n=36)、with normoalbuminuria (n=40) and health control (n=40) (P<0.05).But only serum miR-150 (p=0.005) and miR-155 (p=0.006) changed significantly between diabetes patients with microalbuminuria (n=36) and with macroalbuminuria (n=30). Compared with the diabetes patients with microalbuminuria, miR-150 and miR-155 both are Lower level in macroalbuminuria group, Respectively,2.3 and 1.5 times. Estimated glomerular filtration rate and urinary albumin excretion rate significantly correlated with serum miR-150 and miR-155 level.And Triglyceride significantly correlated with serum miR-155 level.Conclusions:miR-150 and miR-155 may be Involved in the process of pathological mechanisms of diabetic nephropathy; serum miR-150 and miR-155 may be regarded as potential biomarkers to diagnosis the occurrence and development of diabetic nephropathy. Targeting miR-150 and miR-155 may be a new approach to treat diabetic nephropathy.