Role Of Combination Of Oxymatrine And Cisplatin In Inhibitation Of Transplant Subcutaneous Sarcomaof Human Liver Cancer In Nude Mice And Effects On Expression Of MiRNA-122 And MiRNA-21

Objective:The aim of this study was to observe the effectsof oxymatrine (OM) combined with Cisplatin (DDP) on the growth of transplant subcutaneous sarcoma of human liver cancer in nude mice and to explore its molecular mechanisms.Methods:The BALB/c nude mice human liver cancer cell strains HepG2 transplantation tumor model was randomly divided into four groups after its successful establishment. The classified groups (8 mice in each group) were: control group, OM group, DDP group and OM and DDP combination group, all of which were administered by the method of intraperitoneal injection. The weight and gross tumor volume of the nude mice in this period were observed and recorded and the weight change curve and tumor growth curve were drawn. After 14 days of continuousadministration,the nude mice were killed, the tumor was weighed and the tumor inhibiting rate was calculated. The expression of mir-122 and mir-21 in the transplantsubcutaneous sarcoma were detected by the method of real-time fluorescence quantification.Results:Both the use of OM or DDP alone and the use of OM combined with DDP can inhibit the growth of the transplantation tumor of nude mice. The tumor inhibiting rate is OM+DDP group (79.1%)> DDP group (75%)> OM group (31.5%), andthe tumor inhabitation effect of OM combined with DDP is significantly higher than that of OM or DDP group (P< 0.05). The average weight of the nude mice is DDP group (17.33g)< OM+DDP group (20.5g)< OM group (27g)<NS group (28.5g). The mind, appetite, activities and other conditions of the nude mice in OM+DDP group are significantly superior to that of DDP group. The result of Real-Time PCR shows that compared with NS group, the expression of the transplant subcutaneous sarcoma mir-21 in OM group, DDP group and OM+DDP group is down-regulated with the obvious degree of OM+DDP group> DDP group> OM group> NS group; mir-122 expression is up-regulated with the obvious degree of OM+DDP group> DDP group>OM group>NS group.Conclusions:1. Whether the use of OM or DDP alone has the inhibiting effects on the transplantation tumor of nude mice and their combination can enhance the tumor inhabitation effects of the single drug on transplantation tumor of nude mice, manifesting the synergistic effect; OM can improve the whole body conditions of the nude mice with transplant subcutaneous sarcoma and can reduce the toxic and side effects of DDP.2. Possible molecular mechanisms of inhibiting effect of OM or DDP on transplant subcutaneous sarcoma of human liver cancer in nude mice are down-regulation of mir-21 expression and up-regulation of mir-122 expression.