| Objective:As we all know, the body’s cells and tissues in the fibroblast growth factor receptor (FGFRs) molecules are receptor tyrosine kinases (RTK) family members.Recent studies have found that fibroblast growth factor signaling and bone development and disease are closely related, including fibroblast growth factor receptor 3 (FGFR3) is one of the main members of the family FGFRs in the body’s bone tissue development plays an important physiological role. The experiment by observing, detecting fractures of denervation of FGFR3 expression and clarify the regulation of nerve fractures and bone reconstruction FGFR3 specific mechanisms in the loss to the expression of FGFR3 activity from the control aspects of the clinical finding of a new after denervation promote bone tissue injury, the body’s self-repair methods.Methods:In this study,96 rats were randomly divided into:the experimental group and the control group, two groups of 48. All of these rats were established animal model of tibial fracture T10 spinal cord injury(SCI) state, then the experimental group by intraperitoneal injection of FGFR3 blocker PD173074, while the control group did not intervene. Were drawn after 3,5,7,14, 21 and 28 days, removal of fracture callus in each 0.5 cm at the upper and lower ends, after decalcification and fixed, made of 10μm slices. Slicing operation includes counting FGFR3-positive cells, the expression of osteoclast cells and apoptotic cells. Another respectively after 1,3,5,7,14,21 and 28 days recording hindlimb behavioral change process, using behavioral BBB score, and 14,21 and 28 days after the shooting X ray as well as 21 days after the biomechanical testing, and finally the related statistical analysis.Result:At different time points after surgery, the experimental group FGFR3 mRNA-positive cell counts and osteoclasts staining was significantly lower than the control group, the difference was statistically significant (P<0.05). In the process of fracture healing process, compared to control group, apoptotic index callus reduction in the experimental group (P<0.05), and has a characteristic time of distribution., and has a characteristic time of distribution. BBB behavioral scores showed the experimental group and control group were statistically significant (P<0.05) at all time points after the fracture. X-ray films showed fracture healing in rats after experimental group accelerated the process in advance. In biomechanics, in the 21 days after surgery, compared with the control rats, tibia biomechanical experimental group decreased significantly, with statistical significance (P<0.05).Conclusions:FGFR3 involved in the body denervation state of fracture healing process of bone formation within the cartilage, a negative regulator of chondrocyte proliferation, which plays a vital role in the healing of the body to rebuild bone and bone growth and development in. |
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