| Objective In this experiment, diabetic rats back deep Ⅱ° burns manufacture refractory diabetic wound healing model. Observe the healing time, comparative analysis, study the effects of insulin bFGF-topical gel release agent for refractory wounds in diabetic microvascular and III collagen expression.Methods Select Medical Experimental Animal Center of Gansu Province (SPF grade) 2-month-old inbred male Wistar rats weighing 250±40g, the selected rat fast intraperitoneal injection of streptozotocin (STZ) induced by diabetes model, then prepare deep II degree burn model,75 two-month-old inbred male Wistar rats (250±40g) were randomly divided into normal rat blank control group A slow-release gel, gel blank diabetic rats in the control group B back in diabetic rats deep II degree burn wounds sustained release of insulin gel control group C, back deep II degree burn wounds in diabetic rats, bFGF slow release gel D group, back deep Ⅱ degree burn wounds in diabetic rats topical application of bFGF sustained release of insulin gel E group, each with 15. Were scalded rats in each group after the first 3d,7d,11d,15d,21d observe and record the total area of the wound (residual wound area), transparent membrane computing back deep Ⅱ degree burn wound healing rate of the rats, formalin-fixed tissue wounds HE staining check back deep Ⅱ degree burn wounds in rats were observed under pathological healing vision. Formalin-fixed tissue wounds underwent rat-CD31/Ⅲ collagen immunohistochemical staining to detect the back wound tissue in rats CD31, the expression of type III collagen Ⅲ. ELISA kit wound in the back of each rat bFGF levels. Detection of sustained-release gel formulation stability conditions in vitro release.Result:bFGF-insulin gel prepared with a suitable release agent adhesion, good moisture resistance, easy to save and apply. Chitosan Gel Detection bFGF-insulin within 30 d to maintain good stability. In addition to the rats after injection of STZ diabetic model modeling died three (already supplement modeling), blood glucose normal rats, diabetic rats group survived to the end of the experiment, the wound did not find obvious signs of infection. Before each group of rats given deep second degree burn wounds interventions within 3 d, no significant changes were observed visually and differences.7,11,15,21 d of observation, bFGF rat insulin wounds heal best sustained-release gel group, B group the lowest rate of healing in rats, E group healing rate is the highest. Subject to section 3d outside the rats back deep second degree burn wound healing rate pairwise comparisons, the results were statistically significant differences (ie:P< 0.05).Histological observation at each time point:the wound tissue microvasculature of the E group, granulation tissue growth and the growth of collagen fibers are better than the rest of the group. After the burn with time, the rats in the wound tissue capillaries, collagen protein Ⅲ gradually increased, in the implementation of interventions on various indicators peaked at 15 d, 21 d Expression intervention significantly decreased the indicators; take all back time after rat wound tissue immunohistochemical staining observed:CD31 positive cells were in the first 3 d after the intervention have been expressed, with time, the wound in the back of the rats number of positive staining cells in tissue gradually increased; 15d starts to decrease from the number of immunohistochemical staining of cells after intervention; each time point D, the number of group E cd31 staining and expression of collagen Ⅲ was significantly better than the other groups, the highest in group E, wound tissue of rats in Group B the index was the lowest, the results of pairwise comparisons between groups, the differences were statistically significant result (ie:P <0.05).Conclusion:1. External use bFGF slow-release system can promote diabetic rats back deep second degree burn wound healing.2. External use bFGF-insulin gel system can promote wound repair of tissue damage and improve wound healing rate;Can promote the synthesis of collagen in wound tissue;Promote wound tissue capillaries, improve blood microcirculation wound organization helps granulation tissue formation. |
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