The Study Of Anti-tumor Effect Of Artemisia Annua And Artemisinin

1 ObjectiveThe antitumor activites of Artemisia annua may become a hot research, it is hope to be developed into a new plant anticancer drug. By the way of animal experiments, to observe the antitumor effect of Artemisia annua, to investigate the possible relationship between these drugs and the body’s immune, in order to provide a reference for the further determine on the mechanism of its anti-tumor. Its next step in research scientific basis for the new use of old drugs to open a research idea.2 Methods2.1 Use UV-visible spectrophotometry to determinate the content of total flavonoids and total polysaccharides in Arteraasia Ethanol Extract and Artemasia Water Extract.2.2 Modes were Sm and H22 bearing mice by subcutaneous injection at the mouse axilla KM mice under SPF was selected and randomly divided into ten groups, they were CTX group, different doses of Artemisinin(150mg/kg, 300mg/kg), Artesunate injection (60mg/kg) and Artesunate Oral(60mg/kg), different doses of Artemasia Ethanol Extract and Artemasia Water Extract(1000mg/kg,4000mg/kg), model control group (Model), each group had 10mice,24h later, each group was given drugs for 10 days. Killed these mice in last given drug 24h later. The tumor, spleen and thymus were weighted after complot dissection to calculate tumor inhibitory rate, spleen index and thymus index.2.3 Modes was EAC bearing mice by subcutaneous injection at the mouse axilla KM mice under SPF was selected and randomly divided into ten groups, they were CTX group, different doses of Artemisinin(150mg/kg,300mg/kg), Artesunate injection (60mg/kg) and Artesunate Oral(60mg/kg), different doses of Artemasia Ethanol Extract and Artemasia Water Extract(1000mg/kg, 4000mg/kg), model control group (Model), each group had 10mice,24h later, each group was given drugs for 22 days. The general situation was observed and survival time in mice was compared, and measured cum survival of mice.2.4 Modes was H22 bearing mice by subcutaneous injection at the mouse axilla KM mice under SPF was selected and randomly divided into ten groups, they were CTX group, different doses of Artemisinin(150mg/kg,300mg/kg), Artesunate injection (60mg/kg) and Artesunate Oral(60mg/kg), different doses of Artemasia Ethanol Extract and Artemasia Water Extract (1000mg/kg,4000mg/kg), model control group (Model), each group had 10mice,24h later, each group was given drugs for 10 days. Killed these mice in last given drug 24h later. The tumor, spleen and thymus were weighted after complot dissection. Preparation of spleen cell suspension with concanavalin (ConA) tumor-bearing mice induced lymphocyte proliferation, MTT assay proliferation of cells.3 result and conclusion3.1 The content of total polysaccharides and flavonoids in Artemasia Ethanol Extract were 12.7% and 18.3%. The content of total polysaccharides and flavonoids in Artemasia Water Extract were 6.4% and 22%.3.2 Artemisinin(ART)、Artesunate(ATS)、Artemasia Ethanol Extract and Artemasia Water Extract can significantly inhibit S180 tumor growth, percentage of antitumor is more than 40%. The groups spleen weight and thymus weight compared with the control group, no significant change. Described above artemisinin murine spleen, thymus and other immune organs no significant effect damage.3.3 In this reasech, Artesunate injection (60mg/kg) can inhibit the growth of sarcom, and no significant toxicity on the immune system. The cum survival time of EAC bearing mice showed:Artesunate injection 60mg/kg doses can significantly prolong the survival time of tumor-bearing mice.3.4 Artemisia annua and artemisinin drugs on lymphocyte proliferation and transformation of the spleen of mice bearing H22 results show that compared with the model group, artemisinin, artesunate, Artemasia Ethanol Extract, Artemasia Water Extract of each dose group were significant differences (P<0.01), prompts can significantly improve tumor-bearing mice spleen lymphocyte transformation function (P<0.01); CTX group was significantly lower than the ability of spleen lymphocyte transformation model group (P<0.01), CTX group inhibit tumor-bearing mice spleen lymphocyte transformation function.