Detection The Mitochondrial Function Of Dendritic Cells From The Children With Acute Leukemia And Literature Review

BackgroundLeukemia is one of the most common children malignant tumor. In acute leukemia,intensive induction chemotherapy induces complete cytomorphological remission in up to 80% of patients. However,because of minimal residual disease,20%-30% patients experience subsequent recurrence of disease.The chemotherapy is critical for the patients with recurrent.So,targeted immunotherapies is essential.The qualities of the most potent professional antigen-presenting cell, the dendritic cell (DC), can be used to stimulate leukaemia-specific cytotoxic T cells. DCs can be loaded with leukaemia antigens, or leukaemia blasts can be modified to express DC-like properties for use in vaccine therapy. Abroad in recent years, the study found that children with acute leukemia have DCs quantity and function defects in the body, this helps to leukemia cell immune escape, eventually leading to anti leukemia immune response of incompetence.But about provide the energy for DCs of mitochondria, its function changes in children with acute leukemia, but has not been reported.ObjectBy detecting the proportion and mitochondrial function of DCs(including myeloid dendriticcells,mDC and plasmacytoid DC,pDC)from the Children with acute leukemia, in order to understand the changes of DC subsets levels and function in acute leukemia children from before to after treatment. That to improve the function of dendritic cells in children with acute leukemia, and to accelerate the DCs vaccine research foundation in immunotherapy.MethodsSelect Children with acute leukemia from qilu hospital pediatric ward (25 cases ALL at diagnosis,19 cases ALL with treatment on the thirty-third day,7 cases ALL recurrence,11 cases AML at diagnosis),extract the peripheral blood,using flow cytometry detect the proportion of CDllc+mDC and CD123pDC in peripheral blood in children,then using JC-1 kits detect the mitochondrial membrane potentia of DCs, to clear their mitochondrial function.Results1.The percentages and mitochondrial function of CD11c+DC and CD123+DC in childhood ALL at diagnosis and recurrence, were significantly reduced than normal controls (P<0.001) and cases with remission (P＜0.01).2.The percentages of CD11c+DC and CD123+DC in childhood with ALL remission were significantly rised than normal controls(P<0.001). However, the mitochondrial function of DCs (including CD11c+DC and CD123+DC) in PB have no significant correlation with normal controls.3.The percentages and mitochondrial function of CDllc+DC and CD123+DC in childhood AML at diagnosis were significantly reduced than normal controls (P＜0.001).Conclusions1.For the children with ALL, the levels and mitochondrial function of mDC and pDC significantly decreased at diagnosis, but increased after treatment. The percentages of DCs of ALL children with Remission increased than normal children, and the mitochondrial function of DCs return to normal, anti-tumor immune response is more active.2.The percentages and mitochondrial function of DCs from AML children at diagnosis and children ALL at recurrence decreased significantly.So for the children with ALL and AML, may improve the mitochondrial function of DC to exert its function of antigen presented.