| Stromal-interacting molecular 1 is a transmembrane protein expressed in endoplasmic reticulum, which could maintain the intracellular calcium homeostasis and regulate the intracellular calcium ion concentration. A large of studies indicate that the change of intracellular calcium ion concentration are related with the differentiation, proliferation, apoptosis, migration of tumor cells. Currently, the relationship between STIM1 and tumors attract a widespread attention. It has report that STIM1 is expressed in a variety of tumor cells, such as breast cancer, cervical cancer, liver cancer, lung cancer. At present, the related reports between STIM1 and gastric cancer have not been found. There are differences in the biological behavior of different tumor cells. ObjectiveThe study aim at investigating the expression of STIM1 in gastric carcinoma. Then observe the impact of STIM1 on the proliferation, apoptosis, migration, invasion of gastric cancer. And explore the mechanism preliminary. Methods(1) STIM1 expressed in four gastric cancer cell lines, MKN45, SGC7901, AGS, NCI-N87, which was detected by Western Blot.(2) STIM1 specific small interfering RNA transfection inhibited the expression of STIM1, which confirmed by western blot and qrt-PCR.(3) Gastric cancer cells proliferation were deceted by CCK8 kits;(4) Cells apoptosis were deceted by Flow Cytometry.(5) The cell adhesion, invasion and migration were deceted by cell adhesion assay and Transwell, respectively.(6) Western blot was used to explore the mechanism of STIM1 and MAPK pathway.(7) STIM1 expressed in gastric cancer vascular endothelial cell was detected by immunohistochemistry.(8) STIM1 expression in different clinical stages of gastric cancer tissues and normal gastric tissues was calculated by immunohistochemistry and western blot. Analysis the relevance of STIM1 and clinical staging, depth of invasion, differentiation degree in gastric cancer. Results(1) STIM1 was expressed in four gastric cancer cell lines. Higher expression of STIM1 was found in SGC7901 and MKN45 than in NCI-N87.(2) The gene and protein of STIM1 expressed in gastric cancer cells knock down by STIM1 si RNA(3) The abilities of cell proliferation and apoptosis of MKN45 and SGC7901 have no change significantly after si RNA transfection.(4) Cell adhesion was enhanced, and the abilities of migration and invasion was suppressed in gastric cancer cells after STIM1 si RNA transfection.(5) The expression of MEK, ERK, p-MEK, p-ERK protein had no change in gastric cell nearly after STIM1 si RNA transfection.(6) STIM1 expressed in tumor vascular endothelial cells.(7) STIM1 were expressed in both gastric cancer tissues and normal gastric tissues, and the positive rate of STIM1 in gastric cancer tissues was higher than that in normal gastric tissues ConclusionSTIM1 expressed both in gastric cancer tissues and normal gastric tissues, but the expression rate in tumor tissue is signi?cantly higher than the normal tissues. STIM1 had no obvious effect on gastric cancer cell line proliferation, apoptosis. The adhesion promotion and migration inhibition in gastric cancer cells were mediated by the down-regulated of STIM1, but the mechanism is still unclear at present. |
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