The Research On Low-voltage Induceed Myocardial Damage And Influence Of Phosphocreatine Disodium Salt And Shuxuening On The Injury

Objective:Through the establishment of low-voltage injury model of rat heart, to observe the changes of cardiac injury in rats on a low voltage of creatine kinase, electrocardiogram, and analyze morphological changes in myocardial tissue and to explore the effect of Phosphocreatine Disodium Salt and Shuxuening Injection of cardiac injury in rats by observing creatine kinase, electrocardiogram, and analyze morphological changes of myocardial.Method:1. Design an model of injured heart caused by low voltage electric force. The model of injured heart caused by electric force was replicated on Wister rats by 50 Hz, 220(150-200 m A) current.Body weight 200±10g of SD male rats were randomly divided into normal group and electric shock injury group(Four subgroups). Electric shock duration is divided into groups based on 25 s, 30 s, 35 s, 40 s group,and each group with 8 rats. With an electric shock on Wister rats chest(heart surface projection position) for 25 seconds,30 seconds,35seconds,40 seconds by 50 Hz,220V(150-200-m A) current.Then Extract all rats abdominal aortic blood and take out the heart tissue from it after 48 hours,and stored respectively the heart into 10% formalin.Check the creatine kinase in each group,and to observe cardiac morphology under light microscopy of HE staining, and shoot to save analysis. 2. Replicate the electric shock 30 seconds model and reseach. Extract all rats abdominal aortic blood and take out the heart tissue from it at different times after electrical injury( 1h, 3h, 6h, 12 h, 24 h, 48 h, 72 h, 96h), and then observe CK, CK-MB, LDH variation and morphological changes in myocardial tissue in HE staining.3. Body weight 200±10g of SD male rats were randomly divided into normal control group,model group, Physiological saline + electrical injury, electrical injury +low-dose phosphate sodium group, electrical injury + middle-dose phosphate sodium group, electrical injury + high-dose phosphate sodium group, electrical injury + Shuxuening injection group, and each group of 6 rats.Normal control group and model group:untake any measures,just common Breed; electrical injury + Physiological saline group:intraperitoneal inject saline 0.5ml after Electrically damaging 2 hours, and 12 hours; Different doses of sodium phosphate group were given intraperitoneal injection low-dose 45mg/kg/d,middle-dose 90mg/kg/d, hypso-dose 180mg/ kg/d after Electrically damaging 2 hours and 12 hours,and the amount of liquid is 0.5ml; electrical injury + Shuxuening injection group were given Shuxuening of intraperitoneal injection 1.5ml/kg/d at the same time,and the amount of liquid is 0.5ml. Then Extract all rats abdominal aortic blood and take out the heart tissue from it after 24 hours,and stored respectively the heart into 10% formalin.Check the creatine kinase in each group,and to observe cardiac morphology under light microscopy of HE staining, and shoot to save analysis.Results: 1. Established a low voltage cardiac injury model in rats successfuly 1.1 Compared with the normal control group,electric shock injury group manifested decreased vitality,irritability,poor appetite,and deterioration in general condition,and CK, CK-MB, LDH levels were significantly higher(P<0.01), the difference was statistically significant.Electric shock lasted 35 s group and 40 s group most of the rats were shot to death during the experiment, or a shorter survival time after the electric shock. compared with Electric shock 25 s group rats,the 30 s group generally manifested at somewhat deterioration in general condition, and CK, CK-MB,LDH levels no significant difference(P> 0.05). 2 Compare with the changes after electric shock injury of myocardial injury in rats at each time point. 2.1 The levels of CK, CK-MB, LDH gradually reach a peak in the first 24 hours,but at 48 hours and 72 hours,the levels were less than 48 hours Point. 2.2 Myocardial morphological changes in the structure under light microscope at each time point.1h-24 h after injury, focal myocardial necrosis, myocardial rupture, ischemia, cardiac striated part unclear, condensation nuclei, stained the nuclear elongation, the nuclear chromatin assembly, nuclear membrane thickening, was empty bubbly; myocardial edema, the gap widened, some areas scattered bleeding; small blood vessels to dilate, congestion, red blood cell aggregation. 24h-96 h after injury, myocardial degeneration and necrosis of the scope to expand; cardiomyocytes stripes unclear, part of the heart muscle cells were dissolved, muscle wire breakage, some cardiac nucleus fragmentation, dissolution disappear; myocardial fine visible small blood vessels to dilate, red blood cell adhesion in the vessel wall, and some small blood vessels visible thrombosis. 3 The changes of serum creatine kinase and HE staining of myocardial about sodium phosphate group and Shuxuening groups 3.1.1 The CK of blood-serum of normal group were discerned 479±21.1U/L, Comparing to the normal control group, The CK of blood-serum of 30 seconds electrical injury model group and model group + saline are discerned 1938±54.7U/L、1945±51.2U/L(P<0.05). The CK-MB of blood-serum of normal group are discerned 283±18.6U/L, Comparing to the normal group, The CK-MB of blood-serum of 30 seconds electrical injury model group and model group + saline are discerned 1145±49.5U/L 、 1150±62.3U/L(P<0.05). The LDH of blood-serum of normal group are discerned 384±35.4U/L, Comparing to the normal control group, The LDH of blood-serum of 30 seconds electrical injury model group and model group + saline are discerned 1037±53.3U/L、999±38.7U/L(P<0.05). 3.1.2 Comparing to 30-seconds electrical-injury model group,the CK, CK-MB, LDH of low-dose,middle-dose and hypso-dose phosphate sodium group were significantly lower. The CK of low-dose,middle-dose and hypso-dose phosphate sodium group respectively are discerned 1278±42.6U/L 、 1153 士 55.2U/L 、 931 士 55.2(P<0.05);the CK-MB of low-dose,middle-dose and hypso-dose phosphate sodium group respectively are discerned 923±22.0U/L 、 782±24.4U/L 、672±30.0U/L(P<0.05);the LDH of low-dose,middle-dose and hypso-dose phosphate sodium group respectively are discerned 666±29.3U/L、778±22.1U/L、648±32.7U/L(P<0.05). 3.1.3 Comparing to 30-seconds electrical-injury model group, the CK, CK-MB, LDH of Shuxuening injection group are discerned 1095±48.5U/L、804±20.5U/L、532±42.7U/L(P<0.05),all lower obvious. 3.1.4 Comparing the CK, CK-MB, LDH of blood-serum among three different doses of Phosphocreatine sodium,there is difference among each group(P<0.05),and the hypso-dose group’ effect to the damage of myocardial surpass the other groups.the middle-dose phosphate sodium group is better than the low-dose group dose. 3.1.5 Comparing the CK, CK-MB, LDH of blood-serum between the middle-dose of Phosphocreatine sodium group and Shuxuening injection group,there is no difference(P>0.05).Comparing the hypso-dose of Phosphocreatine sodium group and Shuxuening injection group,the hypso-dose group’effect to the damage of myocardial surpass the Shu Xue Ning injection group. 3.2 The changes of the HE staining of myocardial about sodium phosphate group and Shuxuening injection 3.2.1 Shuxuening injection group: cardiomyocytes stripes unclear, part of the heart muscle cells were dissolved, muscle wire breakage. 3.2.2 Sodium phosphate low and middle dose groups: focal myocardial degeneration, nuclear condensation, stained; cardiomyocytes gap widened, stripes unclear. 3.2.3 Sodium phosphate high dose groups: focal myocardial degeneration, nuclear condensation, within the visible part of the small blood vessels thrombosis,and not seen obviously congested vessels.Conclusion: 1 This experiment successfully build low voltage electric shock model in rats of cardiac injury. 2 The serum enzymes CK, CK-MB, LDH level rises to some degree and the morphology of myocardial tissue is damaged after electric shock injury. 3 Creatine phosphate sodium and Shuxuening injection can reduce the level of the blood of serum enzymes CK, CK-MB,LDH in rats and reduce the damage after electrical in rats to a certain extent From the morphology.this can preserve myocardial damage of electrical injury.