The Effect Of In Vivo Use Of Multi-wall Carbon Nanotubes On Murine Immune Response And Leukemogenesis

Purpose:To explore the biological safety of carbon nanotubes by studying the impact of carbon nanotubes on mouse leukemia model, the influence on leukemogenesis after immune function changes, and the relevant molecular mechanisms.Methods:1) Preparation of oxidized water-soluble multi-walled carbon nanotubes;2) Establishment of mouse leukemia model;3) Subcutaneous injection of carbon nanotubes into immuno-competent mice to observe the incidence, record the survival time, and compare the survival rate and reveal the effects of carbon nanotubes on leukemia;4) Detection by FACS of the subtype of leukemia induced by MOL4070 virus;5) Subcutaneous injection of carbon nanotubes to rapamycin-suppressed and immune-deficient mice to reveal the relationship among carbon nanotubes, immune response and leukemogenesis;6) Detection of the levels of pro-inflammation cytokines in the peripheral serum of mice by ELISA;7) Detection of the levels of pro-inflammation cytokines in the supernatant of RAW264.7 treated with carbon nanotubes by ELISA.Results:1) Successful preparation of the oxidized water-soluble multi-walled carbon nanotubes;2) Successful establishment of the mouse leukemia model;3) The leukemia type induced by MOL4070 virus was mostly myeloid leukemia,followed by lymphoid and mixed leukemia;4) The survival rates of leukemia mice injected with carbon nanotubes were different in leukemia mice under different immune status;5) The levels of pro-inflammation cytokines injected with carbon nanotubes were different in leukemia mice under different immune status;6) The survival rates of leukemia mice received different times of injection with carbon nanotubes were different;7) Carbon nanotubes could promote the release of pro-inflammatory cytokines from RAW264.7 cells.Conclusions:Carbon nanotubes could accelerate the leukemogenesis in immuno-competent mice, but they had no apparent effect on the leukemia mice with immuno-suppression or immuno-deficiency. The findings may be due to the chronic inflammation characterized by the release pro-inflammatory cytokines.