Primary Study Of The Effect And Mechanism Of Curcumin And Imatinib On The Proliferation And Apoptosis Of Chordoma Cells

Objective To observe the inhibitory effect and mechanism of Curcumin in combination with Imatinib on proliferation and apoptosis in chordoma cells.Methods The chordoma cell line CM-319 was cultured in vitro and treated by different concentration of Curcumin and(or) Imatinib for 24~72h. Proliferation of cells were detected by WST-1 methods, expression of m RNA of platelet-derived growth factor receptor-β(PDGFR-β) was detected by real-time PCR; Phosphorylation of Akt and expression of protein of caspase-3,bcl-2 and PDGFR-β were measured by Western blot. Apoptosis of cells was detected by flow cytometry.Results 1、As the concentration of Curcumin and(or) Imatinib increased, the inhibitory effect on CM-319 proliferation was also gradually strengthened. Then the survival rate reached to 50%, the combination index was 0.86. 2、10μmol/L of Curcumin could not decrease the expression of PDGFR-β m RNA of CM-319 cells, but could inhibit the expression level of PDGFR-β protein. As the increase of curcumin concentration, the expression level of m RNA and PDGFR-β was gradually decreased. The expression of PDGFR-β m RNA and protein was not affected by treatment of 1μmol/L of Imatinib, but it could significantly decrease PDGFR-β m RNA and the expression level of protein after used in combination with Curcumin for 24 h. 3、Co-incubation of 1μmol/L Imatinib and 10μmol/L Curcumin could decrease the level of the phosphorylation of Akt. 4、1μmol/L of Imatinib and 10μmol/L of Curcumin had no effect on CM-319 apoptosis, the number of CM-319 apoptosis only slightly increase after deal with 20μmol/L Curcumin, when the Curcumin was used in combination with Imatinib, the apoptosis of cells proportion was significantly increased.Meanwhile, level of caspase-3 was rised obviously,but level of bcl-2 was decreased.Conclusion 1. It is probable that Imatinib and Curcumin can inhibit chordoma cells proliferation by inhibition of the expression of PDGF-β, phosphorylation of PI3K/Akt. 2. Combination of Imatinib and Curcumin can induce chordoma cells apoptosis induced by down-regulation of bcl-2 and up-regulation of caspase-3.