The Human Umbilical Cord Mesenchymal Stem Cells For The Treatment Of Radioactive Intestinal Damage In Rats

Objective: Explore the human umbilical cord mesenchymal stem cells on rat intestinal radioactive damage whether has the therapeutic effect.Method: Thirty six healthy female SD(age of 1.5 months, weight(200±20g) rat, were randomly divided into three groups and each group has twelve rats, the groups are blank group, irradiated group and experimental group. Blank group: Don’t irradiate, in the experiment on the second day rats’ caudal vein injected 1ml normal saline. Irradiated group: The experiment on the first day, use the total dose for 9Gy’s 60Coγ ray to irradiate rat’s whole abdomen, the next day rats’ caudal vein injected 1ml normal saline. Experimental group: The experiment on the first day, use the total dose for 9Gy’s 60Coγ ray to irradiate rat’s whole abdomen, the next day injected 1ml human umbilical cord mesenchymal stem cells(number about 106). Seven days after the experiment, each group rat’s abdomen injection of chloral hydrate to be put to death, and collect heart blood and intestinal tissue. Detection of serum IL-10、TNF-α and the content of NO in the organization to understand the anti-inflammatory effect. Through the methods of immunohistochemical staining in rat intestinal Ki67 positive cells are marked to understand the repair of the intestinal epithelial tissue, collect the specimens under light microscopy to observe intestinal tissue pathologic changes.Result:1. Each groups of IL-10、TNF-α、content of NO change display: Compared with irradiation group, experimental group has a higher IL-10 contents(P<0.05) and lower NO and TNF-α(P<0.05) contents. Compared with irradiation group and experimental group, blank group has a higher IL-10 contents(P<0.05) and lower NO and TNF-α(P<0.05) contents. Reflecting that the experimental group‘s inflammatory damage degree is lighter irradiation group.2. Each group’s rat small intestinal Ki67 positive cells markers display: Blank group rat’s Ki67 positive cells significantly more than in the irradiation group and experimental group, P<0.05; The experimental group rats’ Ki67 positive cells increased significantly than in the irradiation group, P<0.05. Show that the human umbilical cord mesenchymal stem cells can promote intestinal proliferation cell number increase.3. Each groups of rat’s intestinal pathological section is visible: For blank group: intestinal epithelial cells of the villus arranged neat and smooth, villus and crypt’s structural integrity at all levels, the intestinal submucosa not seen blood vessels dilate and congestion. Compared with blank group, intestinal epithelial cells of the villus in irradiation group and experimental group arranged irregular, can see part of the villus structure incomplete or even fall off, intestinal gland degeneration and necrosis, intestinal crypt structure changed, capillary blood congestion. Compared with irradiation group, intestinal mucosa villus edema is lighter than in experimental group, fall off a small amount of epithelial cells, most of the glands and crypt structure normal, blood vessels expand is lighter.Conclusion: The human umbilical cord mesenchymal stem cells in the treatment of SD rat intestinal after radiation injury, NO and TNF-α content decreases, IL-10 content increase, small intestinal proliferation Ki67 positive cells increased, reduce the pathological damage.