| Serratia marcescens were isolated from the celom of died Locusta migratoria manilensis(L. m. manilensis) by artificial breeding in Hebei Province. The main biological research including morphology, biological characteristics, phylogeny content, etiology significance, serotyping, PFGE molecular patterns and so on.In this study, the different strains of Serratia marcescens had the same phenotypic characteristics, but in the strains exists no red pigment producing strain. Through the experiment of the artificial infection and judged the pathogenic significance by "Koch postulates", the divided strains had been certificated strong pathogenicity, and it was the primary pathogen why locust dead.Selected a representative strains of Serratia marcescens which isolated from the different locusts farms, used the classic serological agglutination test method which serological homology analysis showed that the homology were different among different strains, but there were significant serological crossreactivity. Used the method of pulsed field gel electrophoresis(PFGE) to molecular classified the different strains, the results revealed the 11 strains of Serratia marcescens in genus was 64%~100%, there were different levels of relationship with each other.The results showed that the wild strain isolated from the breeding locusts, with typical biological and phylogenetic characteristics of Serratia marcescens, but there were different antigen specificity, PFGE existed some relationships between them, for the artificial breeding of locusts had an important etiologic significance. In addition, the study, not only could consummate the biological characteristics of Serratia marcescens, but also provided scientific information for examining the Serratia marcescens as a reference value. On the other hand, the Serratia marcescens was not belong serious pathogenic bacterias for humans and farm animals, it would provide insights for the research on biological control of locustas. |
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