| Objective: To further validate the control of H2 S in bone metabolism and preliminarily studied that whether the control ability of H2 S in bone metabolism plays a significant role in the treatment of osteoporosis based on the index measuring of bone mineral density(BMD), biomechanical index and some simple physical indicators.Method: Dividing 48 non-pregnant SD rats of SPF with the age of 6 months into 4 groups with 12 rats in a group. They are control group(bilateral ovaries observation only by exploratory operation), operation group(surgical removal of bilateral ovaries), low doses of Na HS injection group(intraperitoneal injection of Na HS lasting for 12 W with the dose of 30μmolkg/d after resection of bilateral ovaries for 12w) and high doses of Na HS injection group(intraperitoneal injection of Na HS lasting for 12 W with the dose of 90μmolkg/d after resection of bilateral ovaries for 12w). Bone mineral density(BMD) of left femur in four groups of rats, weight, femoral wet weight, diameter measurement of femoral midpoint of the femur and three point bending mechanical test are conducted after lasting for a total of 24 weeks. Observe and analyze the differences in these rat indicators between groups.Result: Compared with relatively simple surgical groups, bone mineral density(BMD) and biomechanical indexes in each project in high doses of Na HS injection group have markedly improved, in which some parameters are close to those in sham-operation group.Conclusion: It is indeed that H2 S can influence the bone metabolism, and increase mineral deposition and improve the biomechanical properties in femur of ovariectomized rats with osteoporosis. |
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