An Observation Research On The Expression Of Estrogen Receptor In Breast Cancer Cell About Their Morphological Characteristics: Using By Atomic Force Microscopy

Objective Observe the morphological changes in surface of breast cancer cell membrane about their different expression of estrogen receptor using the atomic force microscope, in order to explore and provide new ideas, sub-cellular level basis and guidance on mechanism of tumor’s occurrence, early and cell’s diagnosis and clinical therapy of tumor.Methods Collected the freshly excised breast cancer tissue in vivo from 2013 in November to 2014 year in July. The tissues were all from Beijing Armed Police General Hospital. Got breast cancer cell by cell printing slice method and then prepared for the AFM scanning under dry and clean environment. Divided the breast cancer cell into two groups according to the expression of estrogen receptor antibody’s results made by S-P immunohisto-chemistry staining from the remaining tissues. The atomic force microscopy was used to observe the surface morphology of breast cancer cell’s membrane characteristics of estrogen positive expression group and negative group. Measured the characteristic parameters of average roughness, the maximum peak height, average maximum depth and surface area difference quantitatively and made different analysis by statistical method.Results The breast cancer cells acquired by printing method were distributed uniformly and suitable for the observation of single cell’s morphological features. The staining of hormone levels could not be distinguished between breast cancer cells by HE staining. After being grouped by immunohisto-chemical staining results, we found the breast cancer cells were round/oval and had no significant difference between the cell’s membrane morphology in the resolution of estrogen positive group and negative group by AFM under a large scanning range such as 30μm. However, with the scanning range being gradually reduced to 5μm or 1μm, cell’s membrane surface ultrastructure was clearly present with the resolution increases. There were obviously difference between estrogen positive expression group and negative group: the breast cancer cell membrane’s surface of positive group was concave convex and uneven, like "tat" uplift, "fine" and “sharp”;while the negative group’s pulift was like “ditch”, ”full” and “thick”. The two groups of cells had the obvious difference in the average roughness, the maximum peak height, average maximum depth and surface area difference(p<0.05).Conclusions The surface structure of the cell’s membrane is different between cells with different functional state. There are big differences in morphological structure of the cell membrane tumor cells under different expression of receptors. It provides a train of thought, new method and basis for making early and cell’s clinical pathological diagnosis, exploring the mechanism of tumor’s occurrence and directing clinical therapy of tumor.