Inhibitory Effects Of MiR-140-5p On Proliferation And Invasion Of Human Glioma Cell Lines U251 And U87MG In Vitro

Part one: Study on expression of mi R-140-5p in human glioma cellsObjective: To study the expression of mi R-140-5p in normal brain tissues and glioma tissues, to explore whether its significance on glioma cell growth and development, which lays a foundation for further study of its function in glioma.Methods: the glioma tissues were collected in Department of neurosurgery operation resection in the First Affiliated Hospital of Soochow University, cultured glioma cell lines U251, U87 MG, SHG44, total RNA was extracted from the tissues and cell lines by Trizol method, the expression of mi R-140-5p detected by Real-time PCR in glioma tissue and cell lines.Results: The expression of mi R-140-5p in normal brain tissues was significantly higher than that of human brain glioma tissues and glioma cell lines(P<0.01), and in the glioma tissues, the higher the pathological grade, the lower of mi R-140-5p(P<0.05).Conclusion: It’s low that the expression of mi R-140-5p in glioma cells, which may play a certain role in the growth, invasion of glioma cells, it is worth further study.Part two: Inhibitory effect of mi R-140-5p on proliferation and invasion and induce apoptosis of human glioma cellsObjective: To study the effect of mi R-140-5p on human glioma cell linesproliferation, invasion and apoptosis,and provide a theoretical basis for the treatment of glioma.Methods: mi R-140-5p mimics were transfected into U251 and U87 MG cells by Lipofectamine 2000. At the same time, the blank control group and negative control group were established. The expression level of mi R-140-5p was identified by q RT-PCR. After transfection, transfection efficiency was observed by fluorescent microscope. The ability of cell proliferation was detected by CCK-8;cell cycle and apoptosis were detected by flow cytometry; scratch assay was used to detect the cell migration ability; the cell invasive ability was evaluated by Transwell assay.Results: After over-expression of mi R-140-5p, the U87 MG and U251 cells proliferation and invasion ability were obviously inhibited. By flow cytometry on cell cycle analysis(P<0.05), results showed that cell cycle was arrested in G0/G1 phase and S phase was decreased. The cell apoptosis was significantly increased after over-expression of mi R-140-5p(P<0.05).Conclusion: Over expression of mi R-140-5p can inhibit U251 and U87 MG glioma cell proliferation, arrest cell cycle, promote its apoptosis, and can effectively inhibit the invasiveness.