Effect Of Gypenosides On The Proliferation Of HMCs Induced By AGEs

Objective: To investigate the effect of gypenosides on the proliferation of human mesangial cells and the expression of TGF-β1 and FN mRNA induced by AGEs, approaching its effective mechanism delaying the progression of diabetic nephropathy, which will be the theoretical basis of Exploitation and Application of Gynostemma pentaphyllum. Methods: Logarithmic growth phase HMCs were divided into blank,model, drug and positive groups,which were induced by AGEs( 200 mg /L) in model, drug and positive groups, drug group was intervened by different doses of GP(25,50,75,100,150,200mg/L) at the same time, while positive group was given 10-1 mmol/L aminoguanidine hydrochloride,blank group was not given any induction and intervention. The inhibition of HMCs proliferation was determined by MTT assay after treated for 24,48,72,96 h. The expressions of TGF-β1 and FN mRNA in HMCs in all groups which the doses of GP was(25, 75, 150,200mg/L) were detected by semi-quantitative RT-PCR after treated for 72 h. Results:MTT results showed that, AGEs could significantly stimulate HMCs proliferation in the model group when compared with blank group(P<0.01); Compared with model group and blank group,the inhibition of cell proliferation in drug group increased in a dose-and time-dependentmanner(all P<0.01). Compared with positive group,the inhibition of cell proliferation decreased in drug group intervened by 25,50,75,100 mg /L GP,while increased by 150,200 mg/L GP, and in a dose and time dependent manner(all P<0.01). Semi-quantitative RT-PCR results showed that, The TGF-β1 and FN mRNA was a little expression in blank group. compared with blank group, the expression of TGF-β1, FN mRNA increased in model group(P<0.01). compared with blank group, the expression of TGF-β1, FN mRNA in drug group increased in a dose-dependent manner( all P<0.01).compared with model group, the expression of TGF-β1, FN mRNA in drug group decreased in a dose-dependent manner( all P<0.01). Conclusions: GP can inhibit the over proliferation of HMCs induced by AGEs,and down-regulates the expressions of TGF-β1 and FN mRNA, So as to retard the development of glomerular sclerosis.