| Objective:Gastric cancer is one of the common gastrointestinal cancer, is China’s highest cancer mortality, about 400,000 people die from stomach cancer each year, the total number of the world’s population of 47.8% in gastric cancer mortality [1]. Surgical resection is the preferred treatment for gastric cancer. But because most of the patients had undergone preoperative lymph node metastasis, making the five-year survival rate of gastric cancer patients less than 40%, a number of advanced gastric cancer patients with unresectable survival rate is lower. So to find a new way to inhibit lymphatic metastasis of gastric cancer is very important. That of human gastric cancer drug interfering-SGC7901 nude mice model, the inhibition of endostatin on human gastric tumors grown in nude mice lymph node metastasis purpose of this experiment. Endostar for broader clinical application to provide a theoretical basis.Methods:Logarithmic phase of growth and good condition of human gastric cancer cells SGC-7901 cells were prepared as single cell suspension. Nude mice were injected subcutaneously in the right armpit 0.2ml, locally significant Picchu after injection. After 7 days, all appeared nude armpit tumor mass, successful model. 11 days after inoculation will have to die 32 mice were randomly divided into four groups(n = 8): control group, saline 0.2ml, once a day; Oxaliplatin 5mg / kg,, once every 48h; Endostar group, 3mg / kg, once daily; endostar + oxaliplatin group to endostar 3mg / kg, once a day, and Oxaliplatin 5mg / kg, once every 48 h. The mode of administration were intraperitoneally administered continuously for 14 days. Every 24 hours daily activities observed in nude mice, diet, bowel movements and so on. Weighed once every 72 hours, with a vernier caliper to measure mass relative tumor volumes of the longest diameter a, shortest diameter b according to the formula V =(a × b ^ 2) / 2 is calculated. After treatment is completed, mice were observed for 7 days, mice were sacrificed to take off the neck, remove the tumor tissue, weighed and tumor inhibition rate used to calculate the weight. The stripping suspicious tumor tissue and lymph nodes with 4% paraformaldehyde. After 24 h, the conventional paraffin sections prepared. By immunohistochemical expression of VEGF-C. Application podoplanin protein as a marker of lymphatic vessels, calculated for each group of lymph node metastasis rate and lymphatic microvessel density. TUNEL assay apoptosis of tumor cells in the tumor tissue.Results:1. The experimental group tumor volume was significantly smaller than the saline group. Endostar alone group and oxaliplatin group larger than endostar oxaliplatin tumor volume. Between tumor volume alone endostar and oxaliplatin group had no significant difference;2. The rate of tumor cell apoptosis: the saline group was 16.38 ± 1.48%, endostar group was 28.17 ± 2.72%, oxaliplatin group 31.45 ± 2.79%, endostar + oxaliplatin group 36.95 ± 2.88%. Each drug interfere with apoptosis rate was significantly higher than the saline group. Endostar group was statistically significant(p <0.05) between the oxaliplatin group, endostar + oxaliplatin group and the saline group differences, and the differences between the oxaliplatin group was not statistically endostar significance.Optical density;3. VEGF-C expression: the saline group was 0.78 ± 0.13, endostar group was 0.39 ± 0.10, oxaliplatin group was 0.79 ± 0.15, endostar + oxaliplatin group was 0.36 ± 0.13. Endostar + oxaliplatin group and the saline group, oxaliplatin between groups was statistically significant(p <0.05) differences. Endostar group and the saline group, oxaliplatin between groups was statistically significant(p <0.05) differences. There was no significant between group and the saline group endostar with oxaliplatin group differences; 4.The positive rate of lymph node metastasis group and endostar endostar oxaliplatin group, the number of metastatic lymph nodes and lymphatic microvessel density was significantly lower than the saline group and oxaliplatin alone group; oxaliplatin group lymphatic metastasis positive rate, the number of metastatic lymph nodes and lymphatic microvessel density with the control group had no significant difference.Conclusion:1. Endostar and oxaliplatin are able to play an inhibitory effect on tumor growth, the effect of endostatin in combination with oxaliplatin significantly better than either drug alone;2. endostar able nascent micro lymphatic tumors and metastatic lymph nodes play an inhibitory effect. Oxaliplatin alone found no effect of Appeal;3. Endostar inhibitory mechanism nude mice lymphangiogenesis may be through inhibiting the expression of VEGF-C in tumor tissue, reducing tissue VEGFR-3 activation, inhibition of VEGF-C, VEGFR-3 signal path to achieve a;4. oxaliplatin alone can induce apoptosis in nude mice, inhibition of tumor growth effect. |
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