| Background Renal cell carcinoma accounts for the second place in the genitourinary system tu mors, and has the first mortality rate among all of them. In recent years, the incidence of renal cell carcinoma is in a steady upward trend. The etiology of renal cancer is still unclear, metastati(?)enal cancer cells are extremely insensitive to chemotherapy、 radiotherapy, and immunotherapy only can help a very small number of patients. At present, surgery remains the primary treatment for kidney cancer, but its high recurrence rate, easy-resistant, easy to transfer characteristics cause great difficulties to the treatment, and those are the main reasons of treatment failure in clinical practice. Therefore, further detailed understanding of the origin of kidney cancer, metastasis and drug resistance mechanism, is the key to improve the therapeutic effect. In previous studies, the cancer stem cells and stem cell-like cells have been isolated and identified from a number of solid tumors. ALDH1 has proven to be cancer stem cell markers, which are closely related to the expression and prognosis, recurrence and metastasis of tumors. Recent studies suggest that ABCG2 expression only in the early stem cells, ABCG2 expression gradually stop with the differentiation of cells, thus it has considered to be an important marker for stem cells. This has an effect of pumped out a variety of anticancer drugs from tumor cells, and plays an important role in terms of cancer multi-drug resistant, it is also known as multi-drug resistance transporter protein. However, whether these two proteins associated with the high recurrence of renal cell carcinoma, easy metastasis and drug resistance,more research is needed to study.Objective Detect the expression of cancer stem cell marker ALDH1 and ABCG2 in human renal cell carcinoma, to analyze the clinicopathological parameters between them, and to explore their role in renal cell carcinoma, development, invasion and transfer.Method A series of 78 cases of renal cell carcinoma patients who underwent surgical treatment with complete pathological data surgically resected specimens during October 2010-December 2013 in the First Affiliated Hospital of Xinxiang Medical College, department of urology. In addition, we also collect the normal kidney tissue samples tissue from the tumor margin than 5cm among the above case (30 cases). All specimens were fixed in 4% neutral formalin, paraffin-embedded, made 4μm thick slices. Detect the expression of ALDH1 and ABCG2 in renal cell carcinoma and adjacent normal kidney tissues by immunohistochemical SABC method, and prognostic relevance of data were analyzed.Results The expression of ABCG2 and ALDH1 was respectively 65% and 80% in the clear cell renal cell carcinoma, in the adjacent normal tissues the expression rates of ALDH1 and ABCG2 was respectively 5% and 12%. The positive expression rate of both of them was significantly higher than normal kidney tissue, significant difference (P <0.05), positive expressions of ALDH1 and ABCG2 in RCCC showed significant correlation with tumor grade and tumor clinical stage and lymph node metastasis (P<0.05), but have no significant correlation with patient’s sex and age, tumor diameter (P> 0.05).Conclusion1. ALDH1 and ABCG2 in the clear cell renal cell carcinoma tissue are highly expressed.The expressions of ALDH1、ABCG2 play an important role in the development of RCCC, can be used as prognostic judgement index. This will provide a new idea to the treatment of renal clear cell carcinoma.2. ALDH1 and ABCG2 in the clear cell renal cell carcinoma tissue show high expression with histological grade carcinoma, closely related to the clinical stage and metastasis.3. ALDH1 and ABCG2 in the clear cell renal cell carcinoma tissue are positively correlated. |
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