The Expression And Role Research Of Gata1 In Browning Of White Adipose Tissue Of Mice

There are two kinds of adipose in the body: White adipose tissue(WAT) and Brown adipose tissue(BAT). The former, storing energy in the form of triglycerides, is closely related to obesity, diabetes and the vascular disease; The latter, providing energy by oxidative phosphorylation, can prevent obesity incidence. Studies have confirmed that, white fat can transforme into the brown fat under the proper stimulation. Therefore, it is of great significance for human healty to clarify the specific mechanism of the browning of WAT.Vascular endothelial growth factor(Vegfa) and its family member Vegfb have been found accumulated in adipose tissue in recent years, and be closely related to the development of adipose tissue and energy metabolism. We established the Tetracycline-Vegfa reversible regulated mouse model, and successfully mediated the browning of white fat. In this process, in addition to the key regulator genes of WAT and BAT such as Leptin,Prdm16, Ucp1, Cidea, Bmp4 and Bmp7. Gata1 was also significantly upregulated. Previous studies focus on GATA1 are mainly about its regulating role in erythroid differentiation, the research on the development of fat is limited. Our main work in this study is just about Whether GATA1 plays a important regulating role in the process of adipose differentiation and energy metabolism.In this study, to recearch the role of GATA1 in the browning of white adipose, we first test its expression characteristics between different adipose tissue. Realtime-PCR results showed the expression of Gata1 in BAT is far higher than it in WAT. Using tetracycline-Vegfa reversible regulating mouse model, we found that in the process of the browning of white adipose Gata1 was marked increase, which was significant negative correlation with Vegfa. We constructed pc DNA3.1(-)-Gata1 and lentiviral plasmid, then made Gata1 exogenous overexpression in different cells. Real-time PCR results showed that in both the 3t3-L1 cell lines and primary fat precursor cells, over-expression of GATA1 can up-regulate the expressions of the same brown fat factor genes, such as Bmp4, Pgc1-α and Cidea; But does not affect the expressions of Bmp7, Prdm16 and Ucp1. Our experiment results reveal that in the process of adipose differentiation GATA1 expression is closely related with brown fat development and the browning of white fat; Its role may be achieved by up-regulating the expressions of Bmp4, Pgc1-alpha and Cidea. Perspectively, data arising from this study may be help to add its biological function, provide a new clue for energy metabolism study, as well as provide a potential target to prevent and control obesity.