MYSL Supplement Improves Exercise Performance And Mitigates Exercise-induced Fatigue Of Mice

Objective:To explore the effect of MYSL supplement composed of Lepidium meyenii, Avena sativa L, Pausinystalia yohimbe, L-Arginine-a-Ketoglutarate on exercise adaptation and exercise performance of mice, as well as the corresponding mechanisms, thereby providing the basis for the development of novel and effective nutritional supplements.Methods: Totally 808-week-old mice with body weight of 22.0 ± 1.0 g were randomly divided into four groups including control group (C), and MYSL low, middle and high dose (L, M and H) groups. The mice were subjected to the adaptive rear in the first week, and then the mice were administrated with MYSL at low, middle and high dose (2.5,5 and 7.5 mg dissolved in 0.5 mL of distilled water each day) for 2 consecutive weeks from the second week. The mice from the control group were administrated with water at the identical volume. After the last administration of MYSL for 2 h, the mice were subjected to the exhaustive swimming with the loaded weight of 5% mouse body weight. After exhaustive exercise, blood samples were collected through the removal of eyeball. Meanwhile, the samples of gastrocnemius muscle, soleus muscle, heart tissue and liver tissue were also harvested. The serum BLA, MDA, SOD and BUN were determined by biochemical assays kits, and the expression levels of autophagy-associated signal pathway proteins such as LC3-Ⅰ, LC3-Ⅱ, AMPK-α, NRF2, KEAP1 and p-AMPK-α in tissue samples were evaluated by Western blotting.Results:After administration of MYSL at low, middle and high doses for 2 consecutive weeks, the exhaustive swimming time of the mice from L, M and H groups was much longer than that of the mice from C group. Meanwhile, compared with C group, the BUN in blood samples of the mice from L, M and H were obviously lower (P< 0.01); similarly, SOD activity of the mice from L, M and H groups were higher than that in mice from C group; in addition, the MDA level in mice from L, M and H group was lower than that in mice from C group. Moreover, according to Western blotting analysis, MYSL could result in the up-regulation of autophagy-associated signal pathway proteins such as LC3-I and LC3-II in skeletal muscle.Conclusion:MYSL can enhance the adaptation of exercise to muscle and improve exercise performance through the activation of autophagy.