Analysis Of Drug Resistance Of Enterococcus Faecalis And Enterococcus Faecium In Midstream Urine Of The Patients With Urinary Tract Infection In Antu Hospital Of Shanghai

Objective To detect the antibiotic resistance and resistant genes of Enterococcus faecalis and Enterococcus faecium in midstream urine of the patients with urinary tract infection in Antu Hospital of Shanghai. Methods Enterococcus faecalis and Enterococcus faecium were isolated from the midstream urine of the patients with urinary tract infection in Antu Hospital from Jan. to Oct. in 2012. detect the same bacteria from the same people, The bacteria were identified by MircroScan-Walkaway40 SI and drug sensitivity test was done by K-B method. Antibiotic resistant genes were also detected by PCR. Results The positive rate of tetM gene was 65.2%, ermB gene was 82.6%, aac(6’)/aph (2’) gene was 100%, ant (6)-Ⅰ gene was 100%, aph(3’)-Ⅲ gene was 100% in the 23 strains of Enterococcus faecalis. 23 strains of Enterococcus faecalis’s drug fast rate for erythromycin, tetracycline, levofloxacin,gentamicin (high concentration), ampicillin, penicillin G, vancomycin, were87%,60.9%,78.3%,56.5%,21.7%,52.2%, and 0. In the 18 stains of Enterococcus faecium, the positive rate of tetM gene was 55.5%, ermB gene was 55.5%, aac(6’)/aph (2’) gene was 100.0%, ant (6)-Ⅰ gene was 94.4%, aph(3’)-Ⅲ gene was 100%.18 strains of Enterococcus faecium’s drug fast rate for erythromycin, tetracycline, levofloxacin,gentamicin (high concentration), ampicillin, penicillin G, vancomycin, werel00%,38.9%,100%,55.6%,100%,100%, and 0 Conclusion The positive rate of antibiotic resistant genes was very high in the enterococcus isolated from clinic which has an increasing tendency of multi-drug resistance.