The Effects Of Electrical Stimulation On Electrophysiology Of Atrium An Pulmonary Vein In Paroxysmal Atrial Fibrillation Canine Model

Objective:1.low intensity right ear stimulation on atrial and pulmonary vein e lectrophysiological characteristics, which proves that the low intensity stimulation of the vagus nerve (LL-TS) can reduce the CANS imbalance, and then inhibit the o ccurrence of AF development.2.studies have shown that, the recurrence of AF abl ation and GP ablation, incomplete, or related to nerve regeneration. Study on the 1 eft and right on the ganglionic plexus ganglionic plexus after ablation, low intensit y of vagus nerve stimulation on right ear, paroxysmal atrial fibrillation canine mod el, changes of atrium and pulmonary vein electrophysiological characteristics, to ex plore the low strength of right ear vagus nerve stimulation, therapeutic value of A F recurrence after ablation.Methods:21 healthy adult beagle dogs were randomly divided into three groups:g roup RAP (rapid atrial pacing 500 bpm, n=7), LL-ST group (right ear stimulation of low intensity rapid atrial pacing +500 bpm, n=7), group ABL (cardiac ganglia a blation+low intensity stimulation right ear +500bpm rapid atrial pacing, n=7). A mong them, RAP is simply a group of left atrial appendage pacing, pacing frequen cy,10%-20% higher than the sinus rhythm and pacing threshold was measured, pul se width 0.5ms, and then to 500bpm,2 times the threshold voltage stimulation of left atrial appendage stimulation 16h and record ECG; group LL-ST:low intensity right ear to stimulate the rapid atrial+500bpm, two metal crocodile clip clip in ca nine right ear, alligator clip is connected with the end of GRASS-88 dual channel electrical stimulator (frequency 20Hz, pulse width 2ms), set the threshold intensity to sinus rhythm slow or minimum output voltage of atrioventricular block, while th e low intensity threshold intensity of 70%, and at the end of each hour to re mea sure the stimulus threshold ABL group; cardiac ganglia after ablation of right ear +low intensity stimulation rapid atrial pacing in +500bpm, given the intervention t ragus, using radiofrequency ablation instrument will heart ganglion plexus (ARGP) on the right and left on the ganglionic plexus (LSGP) ablation, until a given stimu lus does not cause Heartbeat plexuses too slow so far. Before and after pacing pac ing in 2h,4h,6h,8h, 10h,12h,14h,16h determination of pulmonary vein atrial a nd atrial fibrillation in different parts of the rate, the duration of atrial fibrillation, effective refractory period (ERP) and effective refractory period dispersion (dERP), and powerlab recorded the right vagus nerve dry nerve signals.Results:1.LL-ST group than in RAP group induced atrial fibrillation was significa ntly lower (P<0.05), the duration of atrial fibrillation was significantly decreased (P <0.05), ERP was significantly prolonged, DERP decreased significantly (P<0.05) at different time.2.ABL+LL-ST group than in LL-ST group induced atrial fibrillation was significantly lower (P<0.05), the duration of atrial fibrillation was significantly decreased (P<0.05), ERP was significantly prolonged, DERP decreased significantl y (P<0.05) at different time.Conclusion:1 low intensity stimulation of the vagus nerve can play a reversal or prevention of paroxysmal atrial fibrillation.2 given cardiac ganglia ablation and lo w intensity of vagus nerve stimulation can further suppress the cardiac ganglion ac tivity, further inhibiting the recurrence rate of atrial fibrillation.