Chondrogenesis Of Synovial Mesenchymal Stem Cells Co-Cultured With Chondrocytes On The Three-Dimensional Scaffold

Objective:Compared to other sources of MSCs, synovial is the closest tissue to articular cartilage, has a higher chondrogenic capacity. Compared to other sources of MSCs, synovial is the closest tissue to articular cartilage, has a higher chondrogenic capacity. By the co-culture, used of the interactions of cells and combined with the scaffold that provided the three-dimensional space will conducive the differentiation of the cells. the aim of this study was take the MSCs and hondrocytes were co-cultured in the chitosan/I collagen and I collagen/PLLA/PDL composite scaffolds, rearch the chondrogenesis and the adhesion of synovial MSCs and chondrocytes co-cultured in three-dimensional space. Methods:The synovial membrane and articular cartilage were harvested from rat knee joint. The synovial mesenchymal stem cells and chondrocytes were obtained through the method of enzyme digestion. The passage 3 synovial mesenchymal stem cells and passage 2 chondrocytes were co-cultured in the chitosan/I collagen and I collagen/PLLA/PDLA composite scaffolds at the ratio of 1:2. Then, the cells/scaffold composite was harvested to be examined morphologically, histologically and immunohistochemically after being cultured 21 days. The confocal laser and Scanning microscope was also employed to detect the cells distribution in the scaffold. Results:After being cultured 72 hours, it could be observed from the two scaffold examined through the scanning electron microscope that the cells adhered on the surface of the scaffold and extracellular matrix surrounding the cells was seen on the scaffold. After being culture d 21 days, it could be found through the confocal laser scanning that the cells were well-distributed on the scaffold, and cells decreased gradually. Type II collagen and toluidine blue staining was positive in the extracellular matrix.Conclusion:This study show that composite scaffold materials can provide three-dimensional growth space for the differentiation of the cells, throughco-cultured, using the cartilage cells secrete growth factors and cellular interactions between synovial mesenchymal stem cells can be induced to the cartilage cell differentiation.