Multifunctional Nanocomposites Intergrate Microfluidic Technology Used For Analysis And Detections

Functional nanocomposites have generally stable physical and chemical properties, and intergrate many properties or functions. They have been widely applied in various scientific research and technology fields due to their advantages of multifunction, easy synthesis and wide ranges of approaches. At the same time, with the rapid development of modern microfabrication, intergration of the functional nanocomposites with microfluidic technology, which contains the superiorities of them have achieved certain periodical results.In this dissertation, we explore the applications of analysis and detection based the platform which combined functional nanocomposites with chip technology. Firstly, we synthesized supeparamagnetic Fe3O4@Au nanoparticle clusters by layer-by-layer assembling approach to form gold layer around the magnetic clusters. Using the technique of soft lithography, we fabricated fast assembling microarrays of superparamagnetic Fe3O4@Au nanoparticle clusters as reproducible substrates for surface-enhanced Raman scattering with the help of the magnetic response of the fabricated nanocomposites. And synthesized upconversion multifunctional nanoparticles and achieved the sensitive capture and detection of circulating tumor cells(CTCs) by intergrating the nanocomposites with rough silicon nanowire arrays based microfluidic technique. Specific research results are as follows:Chapter 1: Classification and application of functional nanocomposites are reviewed in this chapter. Characteristics of microfluidic technology and applications of the platform which combines chip technology with functional nanocomposites were also introduced.Chapter 2: Fast assembling microarrays of superparamagnetic Fe3O4@Au nanoparticle clusters as reproducible substrates for surface-enhanced Raman scattering. We synthesized Fe3O4 nanoparticles which had uniform diameter by hydrothermal approach, and achieved the growth of gold by modifying the magnetic nanoparticles with DA. Utilizing the characteristic of the magnetic response of the superparamagnetic Fe3O4@Au NCs, we fabricated microwells array silicon-based substrates for stable and reproducible SERS detection. At the same time, hydrogel-based SERS substrates were also created via soft lithography quickly and facile. In addition, simulation with the method of finite-difference-time-domain(FDTD) has successfully been applied to calculate the distributions of EM-field enhancement around Fe3O4@Au NCs assembled by our approach.Chapter 3: Capture and detection of circulating tumor cells(CTCs) with upconversion multifunctional nanoparticles and silicon nanowire arrays based microfluidic technique. CTCs are captured by antibody labeled MFNPs and enriched under the magnetic field when following through the microfluidic channel, in which Si nanowire array substrate is incorporated to increase the capture efficiency. Highly efficient CTC capture and detection is then demonstrated not only with artificial samples, but also with clinical samples collected from cancer patients.In summary, this thesis illustrates the applications of analysis and detection of the platforms incorporated functional nanocomposites with microfluidic technology. Our results greatly promise further explorations of those applications of the special platforms.