Study On The Effects Of Some Liquor On Fasting Blood Glucose Of Rats And The Analysis Of Mechanism

Objective: To explore the changes of fasting blood glucose, Ins expression in pancreatic islet B cells, Glu expression in pancreatic islet A cells, serum Ins levels and serum C-P levels in rats throught a brand 54° and 38°consumption of liquor intake of different time period and different dose, attempting to offer certain theoretical basis for studying healthy and scientific drinking. Methods: 90 normal male adult SD rats were randomly and equally divided into normal control group and experimental group.(1)The 54°experiment group(45 rats) were divided into 4 weeks group(4w), 8 weeks group(8w), 12 weeks group(12w). Each group of rats were divided into low dose group(L dose,0.8ml/kg·day), middle dose group(M dose,1.6ml/kg·day) and high dose group(H dose,2.4ml/kg·day).(2)The 38°experiment group(30 rats) were divided into 4w group and 12 w group. Each group of rats were devided into L group,M group and H group.(3)There were 5 normal control rats in 4w group,8w group and 12 w group respectively.Gavage the rats with liquor twice a day, one in the morning and the other in the evening,while the normol comparison group remain untouched.Then detect the fasting blood glucose,the excisions of the pancreas tails and serum removed on the end of 4 weeks, 8 weeks and 12 weeks. Tissues were assessed by immunohistochemical SABC, serum radioimmunoassay, image analysis and morphometry methods in the study. Result: 1. 54°group:(1) There was no statistical significance on fasting blood glucose level between experiment group and normal control group(P>0.05).(2) Serum Insulin radioimmunoassay results: As compared with normal control group, the serum Insulin levels of the 8w(H) group and 12(L) group were increased(P<0.05),with no significant changes in other group.(3)Immunohistochemistry result: Under the light microscope, the immunoreactive products of Ins positive cellsand Glu positive cells were all brown particles in the cytoplasm.①As compared with normal control group,the8w(H) group and 12w(L) group immune staining intensity and the mean density of Ins positive cells were increased(P<0.05),with no significant changes in other group. ②As compared with normal control group, the 12w(L) group immune staining intensity and the mean density of Glu positive cells were increased(P<0.05),with no significant changes in other group.(4)Morphological measurement results: There was no statistical significance on the NA of Ins positive cells and Glu positive cells between experiment group and normal control group.As compared with normal control group, all the experiment group were not statistically significant(P>0.05). 38°group:(1) There was no statistical significance on fasting blood glucose level between experiment group and normal control group(P>0.05).(2) Serum C-P radioimmunoassay results: As compared with normal control group, the serum C-P levels of 12(L) group were increased(P<0.05),with no significant changes in other group.(3)Immunohistochemistry result: Under the light microscope, the immunoreactive products of Glu positive cells and Glu positive cells were all brown particles in the cytoplasm. There was no statistical significance on the mean density of Ins positive cells and Glu positive cells(4)Morphological measurement results: There was no statistical significance on the NA of Ins positive cells and Glu positive cells between experiment group and normal control group(P>0.05). Conclusion: The administration by gavage of this brand 54°and 38° consumption of liquor with the dosage and the time period in this experiment had no significant effect on fasting blood glucose, Ins expression in pancreatic islet B cells, Glu expression in pancreatic islet A cells, serum Ins levels and serum C-P levels of rats.