Effect And Significance Of SDF-1/CXCR4 In Proliferation, Migration And Invasion Of Colorectal Cancer Cells SW480

Objective To discuss the influence and significance of stromal cell-derived factor 1(SDF-1) and its specific receptor CXC chemokine receptor 4(CXCR4) on SW480 colorectal cancer cells′ proliferation, migration and invasion ability. To provide reliable evidence for searching more effective diagnosis and treatment markers and seek possible targets of clinical targeted therapy.Methods The colorectal cancer cells SW480 in logarithmic phase were divided into four groups: control group(without any processing), SDF-1 group(added 100 μg/L SDF-1), SDF-1+1 mg/L AMD3100 mixed group(be the first to add 1 mg/L AMD3100 to incubate for 2 hours, then added 100 μg/L SDF-1), AMD3100 group(added 1 mg/L AMD3100).Immunocytochemistry was used to detect the CXCR4 protein expression in SW480 cells; The expression of CXCR4 m RNA in SW480 cells before and after SDF-1 and AMD3100 was detected by RT-PCR; MTT assay was used to test the change of SW480 cells′ proliferation ability before and after SDF-1 and AMD3100; Transwell chamber migration and invasion experiments were used to test the change of SW480 cells′ migration and invasion ability before and after SDF-1 and AMD3100 separately.Results In colorectal cancer cells SW480, the positive rate of CXCR4 protein overexpression was 80%. CXCR4 m RNA expression was positive in SW480 cells. The expression level of CXCR4 m RNA was upregulated by SDF-1(100 μg/L) which could be inhibited by AMD3100(1 mg/L); The proliferation activity of cells in SDF-1 group(0.847±0.039) was higher compared to those in control group(0.624±0.011) and SDF-1 + AMD3100 mixed group(0.607±0.016); AMD3100 group′s(0.456±0.031) was lower than in control group and SDF-1+AMD3100 mixed group(F=108.0,P<0.05).The number of transmembrane cells in SDF-1 group(98.7±5.8, 33.7±6.2) in transwell chamber experiment was more than in control group(21.0±2.2, 6.1±2.3), SDF-1+1 mg/L AMD3100 mixed group(18.5±8.4, 8.5±2.8) and AMD3100 group(12.1±3.2, 2.1±1.0). However, there was no statistical difference among the latter three groups.Conclusion Colorectal cancer cells SW480 express CXCR4 receptor highly. The SDF-1/CXCR4 biological axis can promote the proliferation, migration and invasion of colorectal cancer cells SW480. It has important clinical significance for the occurrence, development and prognosis of colorectal cancer. And this process can be suppressed by CXCR4 specific antagonist AMD3100.