Effect Of Genistein And Quercetin Exposure During The Prepubertal Period O Ovarian Development And Reproductive Endocrinology Of Mice

Phytoestrogen is a class of aromatic sterols distributing in plants widely. They and their metabolites can bind to estrogen receptors in mammals, and play a similar role of the endogenous estrogen. But it can also play an anti-estrogen effect which depends on the concentration of endogenous estrogen. Because of its unique estrogen-like effect, many researches about their physiological functions had been done in domestic and foreign countries, such as anti-oxidant, anti-aging, anti-cancer and so on. Furthermore, their functional food had been consumed widely. However, the effective dose and the negative effects are not clear.In order to assess the effect of phytoestrogen on human health, we selected the ovary and ovarian cancer cell as targets, and genistein and quercetin as drug to investigate the relationship between flavonoid phytoestrogen and ovarian cancerous development, and the results would provide the data base and theory guideline for the risk assessment work of phytoestrogen in food.The followings are the main contents of this study:1. The relationship between quercetin and genistein with ovarian tumorigenesisBased on the drug concentration of daily intake, the followings parameters were investigated the kinds of physiological changes of ovary in mice and the changes of six types of sex hormones in mice’s plasma after expose to genistein and quercetin with50days and the relationship between these changes and ovarian tumorigenesis from several aspects such as morphology, hormone receptor and so on. The results showed the organ index of ovary had increased, and the basic follicles (primordial follicle, primary follicle, secondary follicle) and the rest follicles (mature follicle, atretic follicle, luteum) had increased and descended respectively, and the positive ratios of PCNA and kinds of hormone receptors had also increased as varying degrees after long-term flavonoid phytoestrogen treatment. The hormones level in plasma of mice were also determined, the results showed that levels of sex hormone (E2, FSH, LH, P, PRL, T) in mice’s plasma had some obvious changes, all these changes involved with ovarian disease, especially with tumorigenesis. Moreover, it was found that the drugs would present their estrogen-like effect through the cumulative effect in ovary with correlation analysis, and the estrogen-like effect of GEN was stronger than QUE through comparing the effects of the two phytoestrogen.2. The relationship between flavonoid phytoestrogen and ovarian tumor progressionBased on daily intake concentration, human ovarian cancer cell SKOV-3was selected as target cell. The effects of daily intake (10-14-10-6M) phytoestrogen effected on development of SKOV-3cell from proliferation and apoptosis were assessed. The result showed that the proliferation rate of SKOV-3had been enhanced markedly, the tendency chart of GEN presented V-like type while QUE presented A-like type. The apoptosis rate had descended after exposure with low dose genistein and quercetin. This result was not similar with a popular point that the estrintreatment could be replaced by phytoestrogen to treatment with cancer, and this dissimilarity would have crucial relationship with the concentration of drug.