| Objective: To investigate the correlation between HPV infection and ESCC in purely Chinese Kazakhpopulations by genotyping both E6and L1genes using MassARRAY technique, and compare the resultsof differert HPV detection methods.Methods:(1) By genotyping both HPV E6and L1genes with MassARRAY technique based on thematrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) wasused to detected HPV infection in89cases of ESCC and NCAT tissues.(2)89esophageal carcinomapatients were analyzed. The association of genotype distribution of HPV with clinic pathological factorsof the carcer was studied.(3) The χ2test was adopted to check the difference in genotype distributionbetween cases and controls by SPSS17.0.Results: With this method, HPV genotypes in89patients with esophageal cancer and49controlsselected from the Kazakh population were evaluated using PCR-MS. Six HPV genotypes (HPV6,HPV16, HPV33, HPV39, HPV51, and HPV82) were present in at least51.7%of the esophagealcarcinoma tissues, which was significantly greater than the28.6%prevalence among controls (P <0.05).HPV16was the most common of all the genotypes that were investigated (HPV16prevalence incarcinoma tissue:49.4%; odds ratio3.02,95%confidence interval1.39–6.53). Moreover, both the totalHPV E6and HPV16E6gene positivity rates were significantly higher in ESCCs than in controls (40.5%vs.20.4%, P=0.017;38.2%vs.16.3%, P=0.008; respectively). The agreement rate between PCR-MSversus both PCR and PCR-RDB assays was moderate agreement.Conclusions: Based on our results, the PCR-MS F method appeared quite suitable for HPV detection informalin-fixed, paraffin-embedded tissues. In addition, our findings raise the possibility that HPV(especially the HPV16type) may be involved in the etiology of esophageal carcinoma in the Kazakhpopulations and that HPV E6gene positivity maybe associated with tumor invasive depth. |
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