Protective Effect And Mechanism Of Sesamin And Vitmin E On Aortal Injury In Rats Induced By D-galactose Combined With Aluminum Trichloride

Objective: To observe the protective effect of sesamin and vitmin E on aortal injury inrats induced by D-galactose combined with aluminum trichloride and explore itsconceivable mechanisms.Methods: Thirty-two aortal injury rats were established by D-galactose（180mg/kg/d,ip）combined with aluminum chloride(15mg/kg/d,ig).12weeks later,aortal injury rats(32) were randomly divided into several groups,including aortic injurygroup (NS5ml/kg,D-gal ip+AlCl3ig), sesamin group (160mg/kg; D-gal ip+AlCl3ig), vitamin E group (VitE10mg/kg; D-gal ip+AlCl3ig), sesamin and vitamin Ecombination group (160+10mg/kg;D-gal ip+AlCl3ig),every group was8respectively.The animals were intragastrically administrated with drugs or NS daily for8weeks.Another eight normal rats as control group, administrated with NS for20weeks.After the last administration, blood pressure was measured by tail cuff；Thethoracic aortic ring of rats was mounted on a bath system, and the relaxation induced byacetylcholine and sodium nitroprusside was measured; The primary pathologic changesof aorta were observed by HE staining; T-AOC, H2O2and NO in serum were measuredby colorimetric analysis; The expression of eNOS possitive cells in aortal weremeasured by immunohistochemistry; The expression of eNOS and NOX4protein inaortal were detected by Western blotting. Results:①Compared with the control group, the blood pressure of model group wassignificantly higher (P <0.01); compared with the model group, the blood pressure oftreatment group were improved, sesamin and vitamin E combination group couldsignificantly reduce blood pressure (P <0.01), and better than the sesamin group orvitamin E group.②Compared with the control group, aortic relaxation response toacetylcholine of model group was significantly lower (P <0.01); compared with themodel group, aortic relaxation response to acetylcholine of sesamin and vitamin Ecombination group was significantly higher (P <0.01or P <0.05), and better thansesamin group or vitamin E group.③Compared with the control group, aorticrelaxation response to sodium nitroprusside of model group was significantly lower (P<0.01); compared with the model group, aortic relaxation response to sodiumnitroprusside of sesamin and vitamin E combination group was significantly higher (P<0.01or P <0.05)，the sesamin group although had no statistical significance (P>0.05),but the trend was slightly stronger than the model group; compared with the vitamin Egroup, sesamin group was statistically significant (P <0.05), sesamin group wasstatistically significant (P <0.05), while the combination group was statisticallysignificant (P <0.01or P <0.05) and with a considerable role in the vitamin E group.④HE staining showed that the aorta of control group was glossy, endothelial cells wereintegrated and packed tightly, the film was no thickening; the aorta endometrialendothelial cells of model group were malalignment, endothelial cell was lost, the filmwas thickening，cell of vascular smooth muscle was hypertrophy and disorganized. The aortal pathological damage of each treatment group had different degrees ofimprovement. Particularly sesamin and vitamin E combination group was moreapparent.⑤Compared with the control group, the level of serum NO and T-AOC ofmodel group were significantly decreased (P <0.01), while the level of serum H2O2wasmarkedly increased(P <0.01); compared with the model group, the level of serum NOand T-AOC of sesamin and vitamin E combination group were significantly increased (P <0.01), while the level of serum H2O2was markedly decreased(P <0.01), and betterthan the sesamin group or vitamin E group.⑥Compared with the control group, theexpression of eNOS possitive cells in aortal of model group was significantlydown-regulated (P <0.01); compared with the model group, the expression of eNOSpossitive cells in aortal of sesamin and vitamin E combination group was significantlyup-regulated (P <0.01) and better than the sesamin group or vitamin E group.⑦Compared with the control group, the expression of NOX4protein in aortal of modelgroup was significantly up-regulated (P <0.01); compared with the model group, theexpression of NOX4protein in aortal of sesamin and vitamin E combination group wassignificantly down-regulated (P <0.01) and better than the sesamin group or vitamin Egroup.Conclusion: Sesamin combined with vitmin E has the following protective effects onaortal injury in rats induced by D-galactose and aluminum trichloride:(1)Reduce thearterial blood pressure of model rats.(2)Improve the aortic endothelium-deriveddiastolic function of aortal injury rats.(3) Improve the aortal pathological damage.(4)Increase the level of serum T-AOC, decrease the level of serum H2O2.(5)Up-regulating the expression of eNOS possitive cells and protein, increase the synthesisof NO.(6) Down-regulating the expression of NOX4, reduce the generation of oxygenfree radicals and play its role of antioxidant effect.