Early Dynamic Expression Of TGF-β₁of Bile Duct Electric Coagulation Under The Stimulus Of Bile

Objective: To observe and compare the TGF-β1of bile duct expression and distributionin the process with or without biliary stimulation under certain time certain parts of acertain intensity electric coagulation of the thermal damage in early healing process,which through the ordinary light microscope. To explore the relationship between thestimulation of bile and the chronic inflammation of bile duct wall.Materials and Methods: Repairing model of electric coagulation of bile duct andmodel of electric coagulation of bile duct in the rabbits were made. The rabbits weredivided into bile stimulus group and no bile stimulus group, each30cases. Each groupwas divided into1d group,3d group,5d group,7d group and14d group afteroperation.Use high frequency operator with20w power, and cause electric coagulation1s, take bile duct at each time point for a TGF-β1immunohistochemical staining andobserve and analyze.Results: According to the normal bile duct wall structure, both bile stimulation groupand no-bile stimulation group can see large areas of gasification necrosis, infiltrationof few inflammatory cells; the bile duct lesion site of3d surgical group can see thefull-thickness necrotic, fibrosis hyperplasia, inflammatory cell infiltration obviously;the change characteristics of bile duct electric injury for the5d operation group arebasically with the same as the third days, but the full layer of fibrous tissue hyperplasiaobviously and the lumen is slightly thickened; the coagulation necrosis changehappened in electric injury bile duct epithelial cells of7d surgical group,around theconnective tissue hyperplasia, both the inflammatory cell infiltration and the Wall thickening obviously;14d operation group of electric injury bile duct epithelial layerstructure can not identify the whole layer of connective tissue hyperplasia, aroundthe obvious, but in contrast, bile stimulation group still visible obvious inflammatorycell infiltration, thickening of the wall. Univariate analysis showed that TGF-β1in eathgroup of postoperative bile stimulation and no bile stimulated group electric point ofbile duct wall, there was a statistically significant difference (bile stimulation group F=186.958, P=0.000; no bile stimulation group F=140.736, P=0000). Further comparisonbetween groups by LSD method found: In addition to the expression intensity of thebile stimulation1d group and3d group(P=0.043), there was no statisticalsignificance.1d group and5d group (P=0.000),1d group and7d group (P=0.000),1dgroup and14d group (P=0.000),3d group and5d group (P=0.000),3d group and7dgroup (P=0.000),3d group and14d group (P=0.000),5d group and7d group (P=0.001),5d group and14d group (P=0.000),7d group and14d group (P=0.000), the expressionwere statistically significant. In addition to the expression intensity of the no bilestimulation1d group and3d group (P=0.035),7d group and14d group(P=0.451) therewas no statistical significance.1d group and5d group (P=0.000),1d group and7d group(P=0.000),1d group and14d group (P=0.000),3d group and5d group (P=0.000),3dgroup and7d group (P=0.000),3d group and14d group (P=0.000),5d group and7dgroup (P=0.001),5d group and14d group (P=0.000), the expression were statisticallysignificant.Conclusion: Whole layer of the bile duct wall electric damage, the range of damage ismuch larger than the naked eye can see, with the time of migration after the injury, thepathological changes of the bile duct heating damage is furious by the tissue cellnecrosis–inflammatory necrotic-coagulation necrosis-the fibrous tissue ofhyperplasia-a wall of fibrous thickening of the development process, Damage at thebile duct wall fibrosis gradually, gradually thickened bile duct wall thickness, bile ductgradually narrow cavity. Fibrosis, hyperplasia, bile duct cavity to reduce the existence of a significant correlation of TGF-β1expression and electric injury early bile ductrepair process, suggesting that high expression of TGF-β1may cause damage in bileduct hyperplasia, and stenosis. Sustained chronic inflammation of bile duct wall causedby biliary stimulation was related closely with the high expression of TGF-β1,fibroblastproliferating actively, extracellular matrix over deposition in the healing process ofbile duct.