Congenital Heart Disease Zebrafish Model Transcriptome Sequencing Analysis

Congenital heart disease is the birth defect caused by abnormalities of cardiac and vascular structure during the fetal period, with complex pathogenic mechanisms. The zebrafish is widely used as an animal model for biological research. Zebrafish embryo is transparent has a fast developmental rate is fast. The phenotype of zebrafish mutant cloche include endocardial loss and heart malformation. Even though cloche is malformed it still can survive, thus making it an ideal animal model for the study of congenital heart disease. Transcriptome sequencing can reveal the overall gene expression, therefor, we used transcriptome sequencing to analyze the gene expression of wild type zebrafish and mutant type zebrafish cloche at30hpf period. We analyzed the differentially expressed genes and their related biological processes and pathways. The analysis found new transcripts and transcribed regions, and provided new information on the improvement of the zebrafish genome annotation. Differentially expressed novel transcripts of the cloche mutation can provide novel insights for further research. We focused on the cloche chrl3deletion region to define the start and stop sites of the deletion region. We analyzed the expression of the pathway genes and interaction genes of the differentially expressed genes in the deletion region, particularly the lycat gene. The results revealed that gene expression changes are caused by the lack of gene expression in the cloche chrl3deletion region.