| Objective Through the cerebral hemorrhage model of rabbits, thispaper explores the different efficacies of stereotactic minimally-invasive hematomaclearing operation in combination with rosiglitazone on removal of intracranialhematoma at different timeframes.It provides experimental basis for clinical selectionof optimal treatment time window of stereotactic minimally-invasive hematomaclearing operation. Methods84New Zealand white rabbits are selected as thesubjects whose weights are2.8-3.4kg (regardless of male or female). Stereotactictechnique is used to construct the cerebral hemorrhage model of autologous arterialblood. After successful modeling, the subjects are randomly divided into modelcontrol group, minimally-invasive treatment group, and minimally-invasive treatment+medicine group, and they are compared with normal rabbits. Related processing isimplemented for each experimental group after6h,12h,18h and24h of the successfulmodeling: Pseudo hematoma removal is performed on rabbits in the model controlgroup; intracranial hematomas are removed from the subjects in theminimally-invasive treatment group; in the minimally-invasive treatment+medicinegroup, not only minimally-invasive hematoma removal is performed, but also2.5mgof rosiglitazone (dissolved in0.5ml of normal saline) is injected into lesions. Threedays after the successful modeling, all rabbits in various groups are put to death.Then, the dry and wet weights of the brain tissues surrounding the lesions of rabbitsfrom different groups which have similar weights are recorded and the brain watercontent is calculated. Western blot is adopted to detect PPARγ receptor of brain issuessurrounding hematomas of rabbits from various groups and the expression of its downstream gene CD36. High performance liquid chromatography (HPLC) isadopted to determine the content of glutamate in perihematoma. Result The PPARγand CD36expression of normal control group compared with model control group、minimally invasive group and minimally-invasive treatment+medicine group,thedifference was statistically significant （P＜0.05）.The most expression wasminimally-invasive treatment+medicine group,the lowest expression was normalcontrol group.The minimally invasive group compared with minimally-invasivetreatment+medicine group,we can find in6h、12h、18h、24h there was statisticallysignificant difference（P＜0.05）.The perihematomal glutamate level and the braintissue water content were significant increasing,between the two group there wasstatistically significant difference（P＜0.05）.Compared with normal control group,theglutamate level of model control group、 minimally invasive group andminimally-invasive treatment+medicine group was significant higher than normalcontrol group.And the perihematomal glutamate level in minimally-invasive treatment+medicine group was significant lower than model control group.The perihematomalglutamate level in minimally invasive group slightly lower than model controlgroup,there was statistically significant difference between the group（P＜0.05）.In theminimally-invasive treatment group and the minimally-invasive treatment+medicinegroup, glutamate content is the lowest at6h; With the progress of time window, thecontent of glutamate also increases; glutamate content reaches the highest level at24h.In the above two groups, there is a significant difference in glutamate contentsbetween any two time windows (P＜0.05).Conclusions Early stereotactic minimally-invasive hematoma clearing operationcombined with rosiglitazone can significantly reduce the brain tissue damagesurrounding acute cerebral hemorrhage of rabbits, the brain water content, and theproduction of glutamate. Its treatment mechanism may be associated withperoxidase-proliferative activated receptor γ in rosiglitazone, which plays a veryimportant role in activating microglia and macrophage to swallow and removenecrotic or apoptotic cells and to reduce oxidative stress. |
PDF Full Text Request