The Study Of Repairing Rat Defective Sciatic Nerve Injury By The Method Of Adhesive Bonding

BackgroundNature disasters and wars frequently happened in recent years, a lot of people wereinjured in these catastrophes, and peripheral nerve injury play a big role among thewounded. In such specific condition, doctors and medical recourse were always limited.Currently, the suture method was the golden standard of repairing the nerve injure, butthis method required long operational time, sophisticated equipments and high skill, soit is not suitable for the above specific condition. Recently, adhesive method coughtmore and more researchers’ attention for operating easily and rapidly. We hadsuccessfully repaired the transectional nerve injury of SD rats in our previous work,with the method of adhesive and modified silicon conduit which inner diamter is1.5mm,and this study is the continuation of our early stage.PurposeTo improve adhesive method, we evaluate the feasibility of repairing the peripheraldefective nerve injury with the methods of cyanoacrylate+modified nerve conduitand+acellular nerve allografts and adhesive+modified nerve conduit, then comparing tothe method of suture+acellular nerve allografts and through this study.MethodA. Modifying the structural parameter of the nerve conduit which had been speciallydesigned for adhesive method.B. Observing the injury severity of the nerve caused by the polymerization heat.C. Testifying the feasibily of this method with animal experiment.Randomly dividethe40SD rats into4groups, A group: repair the nerve with cyanoacrylate, nerveconduit and acellular nerve allografts; B group: repair the nerve with suture andacellular nerve allografts, C group: repair the nerve with adhesive and nerve conduit; Dgroup: do not repair the injury nerve as control group. Drafting the procedures of each groups. Timing the operation, and12weeks after surgery, evaluate the effect by themethod of general observation, Cat Walk system, electromyography, wet weight ratio ofgastrocnemius muscle and histopathological analysis.ResultsA. We will insert5mm nerve ends into the conduit to avoid the glue invade the nerveends, as the nerve defective length is1cm, so the length of the conduit is2cm, and thelength of non-continued channel on the conduit surface is4mm.B. The polymerization heat released by20ul cyanoacrylate can corrode theepineurium but not injure the neural fiber. So one drop cyanoacrylate is safe when weconduct the operation.C. Define the operational procedures of each groups. Timing the operation fromcutting off the nerve to finishing the anastomosis. C group takes3.1±0.5min, is theshortest, A group takes3.5±0.4min, and B group takes11.2±0.3min, three times longerthan C group, through independent-samples T test, Pac<0.05, Pbc<0.01,Pab<0.01.There are not successful repaired nerve in D group.In the other three groups, there areno statistic difference among the results of limb touchdown presure, electromyography,wet weight ratio of gastrocnemius muscle. Through histopathological observation, all ofthe samples have the neural fiber pass through the anastomosis in A,B,C group.ConclutionIn this study, the successful repair nerve injury with cyanoacrylate proves thefeasibility of adhesive method. This method can greatly shorten the operational time,reduce the operational diffculty, more suitable for the specific medical condition thantranditional suture method. Sucessfully repair the defective nerve injury with adhesive，conduit，acellular nerve allografts will contruibute to repair longer defective nerveinjury with adhesive method,and be good for making this technique used into clinic nearfuture.