Based On MMP-9Signal Path To Explore Kidney Invigorate The Circulation Mechanism Of Traditional Chinese Medicine For The Treatment Of Myocardial Infarction Is Studied

Experimental study onObjectiveBased on matrix metalloproteinases-9(MMP-9) mediated signal pathway, explore thekidney invigorate the circulation of Chinese traditional medicine mobilization of rat bonemarrow endothelial progenitor cells (EPC), the molecular mechanism of repair endothelialfunction.MethodsLigation method to establish myocardial infarction model in rats,30rats wererandomly divided into building success kidney invigorate the circulation of Chinesetraditional medicine high dose group (group H) and kidney invigorate the circulation oflow dose group (group L) and model group, each group of10, only take another10normalrats for blank group group (K).Kidney invigorate the circulation of high and low dosegroup, respectively with kidney invigorate the circulation of Chinese traditional medicineaccording to the3g/kg,1.5g/kg plus4ml saline lavage,1times a day.Blank group andmodel group use saline4ml lavage1times a day.4weeks after bone marrow, peripheralblood and ischemic myocardial tissue EPC cultivation,7d after collecting adherent cells byflow cytometry instrument identification of CD34/CD133phenotype, nitrate reductiveenzymatic nitric oxide (NO), ELISA method to detect tissue fiber dissolving enzymeactivators (t-PA) and vascular endothelial growth factor (VEGF) and stromal cell derivedfactor (SDF-1ɑ) and Western blot method to detect matrix metalloproteinases-9(MMP-9)and water-soluble Kit ligand (sKitL). Results1.Bone marrow EPC phenotypic characterization M group expression of CD34/CD133positive rate rise in the K group (P<0.05)H,L,peripheral blood and bone marrowischemic myocardial expression of CD34/CD133positive rate were higher than in Mgroup (P<0.0or P<0.05);And H group is significantly higher than L group (P<0.01).2.The bone marrow, peripheral blood and ischemic myocardial EPC number M isbone marrow EPC number K group increased significantly (P<0.05)M group of peripheralblood and ischemic myocardial EPC number compared with K group, no significantdifference (P>0.05);H, L, peripheral blood and bone marrow ischemic myocardial EPCnumber significantly higher in the group M, K group (P<0.01), H a L group is moresignificant (P<0.01).3.The peripheral blood and ischemic myocardial EPC that NO supernatant fluid, Mgroup of peripheral blood t-PA content and ischemic myocardial NO levels were lower inthe K group (P<0.05);H group, the content of NO and t-PA L group were significantlyhigher than that of K group (P<0.01or P<0.05);And group H content of peripheral bloodand ischemic myocardial NO more pronounced in the L group (P<0.01).4.The bone marrow and peripheral blood of VEGF, SDF-1ɑ peripheral blood level ofexpression of VEGF expression in M group was obviously higher than that of K Group(P<0.05);H group, L group bone marrow and peripheral blood of VEGF and SDF-1expression levels were significantly higher than that of group M (P<0.01);And group Hincreases more significantly (P<0.01).5.The bone marrow and peripheral blood EPC MMP-9immunoblot banding analysisEPC MMP-9M group of bone marrow and peripheral blood protein expression weresignificantly higher in the K group (P<0.05);H and L group of bone marrow and peripheralblood EPC MMP-9protein expression were significantly higher in the M group(P<0.01);And group H increases more significantly (P<0.01or P<0.05).6.The bone marrow and peripheral blood EPC sKitL immunoblot banding analysisEPC sKitL M group of bone marrow and peripheral blood protein expression weresignificantly higher in the K group (P<0.05);H and L group of bone marrow and peripheralblood EPC sKitL protein expression were significantly higher in the M group (P<0.01);Hand L group was no significant difference (P>0.05).ConclusionKidney invigorate the circulation of Chinese traditional medicine can activate MMP-9signal path, increasing its upstream signal VEGF and downstream signal SkitL expression level, thereby EPC mobilization of bone marrow into the blood circulation, repair thedamaged endothelial play a role of "transplant" itself. Clinical researchObjectiveObserve kidney invigorate the circulation of traditional Chinese medicine (TCM) inpatients with acute myocardial infarction (AMI) serum levels of MMP-9, provide the basisfor prevention and treatment of coronary heart disease (CHD) of traditional Chinesemedicine.MethodsSelect36cases of AMI patients were randomly divided into kidney invigorate thecirculation of Chinese traditional medicine group and the control group,18cases in eachgroup.Kidney invigorate the circulation of Chinese traditional medicine group was givenconventional western medicine with kidney invigorate the circulation of traditionalChinese medicine therapy, the control group given conventional western medicinetreatment, a total of2weeks.Observed two groups of TCM symptoms integral,electrocardiogram (ecg) and MMP-9, the changes of c-reactive protein (CRP).Results1.The comparative effectiveness of syndromes kidney invigorate the circulation ofChinese traditional medicine group efficiency33.33%, the total effective rate was88.89%;Control efficiency of16.67%, the total effective rate was55.56%.The rank andinspection, two group total effective rate had significant difference (P<0.05).2. Electrocardiogram curative effect comparison of kidney invigorate the circulationof Chinese traditional medicine group efficiency38.89%, the total effective rate was83.33%;Control efficiency of11.11%, the total effective rate was61.11%.The rank andinspection,two group total effective rate had significant difference (P<0.05).3. The MMP-9change after2weeks comparing kidney invigorate the circulation oftraditional Chinese medicine treatment group expression of MMP-9levels compared withbefore treatment, significantly reduced (P<0.01);Compared with the control group aftertreatment, significantly decreased (P<0.05). 4.CRP changes comparing kidney invigorate the circulation of the traditional Chinesemedicine treatment group after2weeks CRP expression levels compared with beforetreatment, significantly decreased (P<0.01);Compared with the control group aftertreatment, no significant difference (P>0.05).ConclusionKidney invigorate the circulation of Chinese traditional medicine can inhibit the AMIpatients serum levels of MMP-9expression level, and can improve clinical symptoms inpatients with myocardial infarction.