Study Of IGF-1Changing In Cerebrospinal Fluid Of Children With Nervous System Disabilities Before And After Neural Stem Cell Transplantation

ObjectiveBy comparing IGF-1levels in cerebrospinal fluid (CSF) before and after the neuralstem cell transplantation in children with mental retardation, to propose further theorybasis of neural stem cells transplantation and a certain approach for the treatment of mentalretardation in future.Methods28hospitalized children with mental retardation in our department received neuralstem cell transplantation from January,2011to January,2012were enrolled in thisstudy(23males and5females, mean aged6.0643±2.9543years). Lateral ventriclepuncture transplantation and lumbar puncrure had been performed in the first and secondweek, respectively. Cerebrospinal fluid samples before each transplantation were collectedand well kept. IGF-1levels in the samples were measured by ELISA.ResultsIGF-1levels before and after transplantation in the28children were statisticallydifferent significantly (P <0.05). The levels of IGF-1in the cerebrospinal fluid after neuralstem cell transplantation were higher. No significant difference was found between malesand females (P=0.706); Among the28patients, short-term efficacy was observed in15children after the neural stem cell transplantation. Their IGF-1level in cerebrospinal fluidincreased significantly after transplantation (P <0.05). In contrast, short-term efficacy wasnot observed in the other13children. There was no significant change of IGF-1level aftertransplantation(P=0.657). As to short-term efficacy and no short-term efficacy, comparisonbetween the two groups showed significant difference (P <0.05). As to comparison amongmild-moderate, severe and profound groups according to the preoperative classification ofseverity of mental retardation, there was no statistical difference(P=0.778).ConclusionsThe IGF-1concentrations in the cerebrospinal fluid of the patients with mentalretardation were is significantly increased after neural stem cell transplantation, especiallyin the patients with short-term efficacy.The results indicated that a strong evidence ofmechanism of neural stem cell repairing neural injury and a treatment direction of children with mental retardation. ObjectiveTo compare IGF-1levels in cerebrospinal fluid (CSF) before and after the neural stemcell transplantation in children with autism and to propose further theory basis of neuralstem cells transplantation for a certain approach to the treatment of autism in future..Methods11hospitalized children with autism in our department recieved neural stem celltransplantation from January,2011to January,2012were enrolled in our study (10malesand1female, mean aged7.6182±3.8502years). Lateral ventricle puncture transplantationand lumbar puncture had been performed in the first and the second week,respectively.Cerebrospinal fluid samples before and after the transplantation were preserved. IGF-1levels in the samples were measured by ELISA.ResultsIGF-1levels had no statistically significant differences in contrast in cerebrospinalfluid (P=0.083). No significant change of IGF-1in the cerebrospinal fluid between thesamples before and after the first transplantation. Among11patients,6children haveshort-term efficacy after the neural stem cell transplantation. IGF-1in their CSF of the6children after the neural stem cell transplantation increased significantly (P <0.05),. Incontrast, short-term efficacy was not observed in the other5patients. There was nosignificant change of IGF-1level after transplantation (P=0.725).ConclusionsIGF-1concentrations in cerebrospinal fluid increased significantly after neural stemcell transplantation in six autism patients with short-term efficacy. It indicated that a strongevidence of mechanism of neural repairment of autism with neural stem celltransplantation and a treatment direction of children with autism.