The Effects Of1.5T Fetal Magnetic Resonance Imaging On The HTERT-infected First Trimester Trophoblast Cell Line Swan71Cell

Objective:Fetal MRI (magnetic resonance imaging), a new imaging technology which offers even higher resolution over a traditional ultrasound platform has been increasingly drawn attention. However, it is banned for the use in the early stages of pregnancy currently due apparently to the lack of the convinced safety research and data. Nowadays, prenatal screening in the early stages of pregnancy is a new challenge for preventing birth defects, so intensive investigation of how MRI influents the embryonic development in the early stage is critically important and required for addressing the potential concern on its safety usage.Thus we propose to comprehensively investigate the influence andmechanism of MRI in human villous trophoblasts after the exposure of MRI in the early stages of pregnancy.Method:1. After exposed at1.5T MRI for60mins, this hTERT-infected first trimester human trophoblast cell line, which we have named Swan71,was measured by WST-1assay for cell proliferation and flow cytometry for cell cycle.2. The submicroscopic organization of the Swan71cells before and after MRI exposures has been studied with high resolution transmission electron microscopy;3. To gain insights into molecular events in Swan71after MRI exposed, we employed a global stable isotope labeled profiling strategy using iTRAQ reagents, followed by2D LC-MS/MS.The proteomes were investigated at4h after exposed at1.5T MRI for60mins.Results:1. Compared with Swan71cells in control group,the cell proliferation was showed significant reduction in a4-day observation after exposed at1.5T MRI for60mins(p<0.01),while cell cycle was not significantly changed(p>0.5).2. Electron microscope:No obvious abnormal changes were seen in pseudo group,but some mitochondrion enlargement were observed after exposed at1.5T MRI for60mins.3. We identified a total of79significantly differentially expressed proteins after MRI exposures, of which49proteins were up-regulated and30proteins were down-regulated, with more than±1.3-fold difference and T test p-value<0.05compared with unexposed cells. The majority of the MRI-related proteins (such as GRAM domain-containing protein1A,Keratin, type I cytoskeletal14,.Histone H3.2.etc.) are involved in the regulation of metabolic process,response to stimulus and developmental process.Conclusion:We found the changes of swan71cell after exposed at1.5T MRI for60mins in a variety of advanced techniques, but whether these changes are associated with embryonic development is still unclear.Overall, our study will shed light on the safety use of MRI for the offspring from the interdisciplinary research, which is expacted to gain insights on and provide a scientific basis for application of MRI in the early stages of pregnancy.