Expression Of β-catenin, CDH1,SHH, ER α And Her-2in The Progress Of Breast Cancer

Human breast cancer is thought to evolve via sequential genetic alterations from benign hyperplasia of mammary duct epithelial cells, through atypical ductal hyperplasia, to ductal carcinoma in situ, invasive tumor confined to the breast, lymph node involvement, and eventually metastases to distant organs. However, the key molecular events in each link of the development of breast cancer have not been explored. Comprehensive molecular profiling studies comparing DCIS and invasive ductal carcinomas have failed to identify tumor-stage-specific signatures.Some reports suggest that the molecular typing of breast carcinoma in situ is similar to the invasive ductal carcinoma, especially the expression of hormone receptors. This phenomenon not only fits with the breast ductal tumor genesis theory, but also can speculate that the molecular events highlighted in the early phase of tumorigenesis, while the advanced stages are focuses on tumor cells invasion and metastasis event.We may meet some rare cases, occasionally, which show the phenomenon that one patient has two or more histological types, including benign ductal epithelial hyperplasia, atypical epithelial hyperplasia, and ductal carcinoma in situ and invasive ductal carcinoma. These cases make the different histological types have same genetic and environmental background, which make it realized to simulate the development process model of breast ductal carcinoma. In such model, the studies of molecular mechanism of breast cancer will be more scientific in theory and persuasion. ObjectiveTo investigate the expression of CDH1, SHHβ, ERα and Her-2in the tumorigenesis and development of breast cancer.MethodThe expression of β-catenin, CDH1, SHH, ERα and Her-2were detected by Immunohistochemistry in5cases of breast cancer tissues. The expression levels of each protein in different histological types were analyzed.Result1P-catenin expression in breast cancer development process showed the upward trend (p<0.05);2CDH1expression in invasive breast cancer was lower than that in benign ductal epithelial hyperplasia, the differences were statistically significantly (p<0.05), the upward trend in the development of carcinoma in situ, low grade and high grade of carcinoma in situ in carcinoma in situ (p<0.05);3the expression of ER a in the development and progression of breast cancer showed the upward trend (p<0.05)4the expression of Her-2in tumor tissues was higher than that in normal tissues (P>0.05);5SHH protein in breast tumor tissue was increased (p<0.05); the expression of SHH in atypical hyperplasia tissues was higher than that of low grade ductal carcinoma in situ (p<0.05).ConclusionBased on the five special cases, this study found that β-catenin was positively correlated with the tumor genesis and development of breast cancer, CDH1, SHH, ER a and Her-2played important roles in breast carcinoma.