The Role And Expression Of TRPV1and CGRP On Intestinal Motility In Rats With Sepsis

Objective:To observe the effects of sepsis on intestinal motility in rats,and to investigatethe role of TRPV1and CGRP on intestinal motility in rats with sepsis.Methods:20male SPF SD rats were randomly divided into two group:group A(controlgroup)and group B(sepsis group).Animal model of sepsis was made by intraperitonealinjection LPS after12h fasting.LPS (10mg/kg)was diluted to1ml with saline.Rats ofcontrol group were injected with saline of1ml.After intraperitoneal injection,keep fastingfor12h.Then rat of each group was gavaged2ml carbon ink, and administered20minbefore executing the rat.The entire small intestinal was removed after ligation of thepyloric ends and the ileocecal junction. Intestinal transit for each rat was calculated withthe following formula:Intestinal transit(%)=(the distance traveled by the carbon ink/the total length ofintestine)×100.One isolated jejunum of each rat,15cm far from the ileocecaljunction,was reserverd for TRPV1and CGRP immunohistochemistry staining.Conbinethe percentage of positive cell staining degree as the final results. Statistical analysis wasperformed by SPSS18.0. Results: Propelling rate of small intestine of group sepsis was significantly lower thanthat of control group,and the difference was statistically significant. Compared with thecontrol group,the expression of TRPV1and CGRP was markedly higher in the sepsisgroup (P<0.05).Conclusion:1After intraperitoneal injection with LPS,a ruffled fur, piloerection,immobility or lethargy could be observed in rats,and change of blood pressure, pulseand breath also can be observed,so intraperitoneal injection with LPS can make a idealmodel of sepsis.2Sepsis can decrease the propelling rate of small intestine.3The expression TRPV1and CGRP in sepsis group were signifcantly higher than incontrol group,so we indicate sepsis may effect intestinal motility via TRPV1and CGRP.