Bioinformatics Analysis Of Hsa-miR-378and Study On The Pattern Of Expression And Regulation In Human Adipocytes

Objective1. The bioinformatics analysis about hsa-miR-378was performed, including transcription regulation prediction and function analysis of target genes, in order to lay foundation and provide the basis for the further studies in human preadipocytes differentiation.2. The difference about the expression of miR-378in the process of differentiation was investigated.3. The alteration of miR-378expression in matured human adipocytes after intervened by adipokines such as TNF-α、IL-6、leptin、resistin was studied.Methods1. Online tools, such as NCBI Mapviewer, UCSC Genome Browser and Promoter scan, were used for prediction of the CpG island, transcription start position (TSS), transcription factor binding sites (TFBS), etc. TargetScan, PicTar and miRanda were used to predict target genes of hsa-miR-378. The intersection of the three results and validated targets from DIANA LAB-TarBase6.0datebase was analyzed by gene ontology and pathway analysis.2. Real-time PCR was used in studing the alteration of miR-378expression in the process of differentiation and after intervened by adipokines.Results1. Results of bioinformatics analysis showed that:hsa-miR-378might have an independent promoter with C/EBP β transcription factor binding sites. The gene ontology analysis showed the predicted target genes were enriched in transcriptional regulation, protein modification,cell differentiation and other biological processes(P<0.01). The pathway analysis showed the target genes mainly involved in4signaling pathways such as TGF-β signaling pathway, Wnt signaling pathway, and8disease pathways such as Thyroid cancer, Systemic lupus erythematosus(P<0.05).2. Expression of miR-378was found to be significantly elevated after differentiation(P<0.05).3. TNF-α, IL-6and leptin upregulated miR-378expression in matured human adipocytes but not resistin.Conclusions miR-378is closely related with human adipocyte differentiation; miR-378might play an important role in the development of obesity and its complications, and might offer a new target for early intervention and prevention of obesity and its complications.