| With the development of global economy and the accelerated pace of life, people’s life in the event of a qualitative change, the fine food of long-term high fat, high protein and triggered a series of metabolic disorders, such as fatty liver, hyperlipidemia, hypertension, hyperglycemia, especially fatty liver is a common. Fatty liver is divided into alcoholic fatty liver (Alcoholic fatty liver disease, AFLD) and non alcoholic fatty liver (Nonalcoholic Fatty Liver Disease, NAFLD), the western countries the incidence rate of NAFLD reached15%-40%, the incidence rate of NAFLD for9%-40%[1] in asia. Non alcoholic fatty liver disease can cause a series of complications, such as pericardial fat deposition, large artery stenosis, hypertension and other cardiovascular and cerebrovascular diseases. The presence of complications mortality also increased with the increase of related control the disease has become the focus of attention, research on the formation and prevention of pathological mechanism of this disease has become a hot spot in medical study. This experiment replicated the non-alcoholic fatty liver by high fat diet method (NAFLD) rat model, to observe the protective effect of blood lipid metabolism, Portulaca oleracea Decoction on NAFLD rat biochemical index, inflammatory factor, liver pathological changes, and to discuss its non alcoholic fatty liver disease (NAFLD) prevention and control effect and the possible mechanismObjective1Observation of purslane water decoction on the rat model of NAFLD body weight, liver weight, liverpathological changes; lipid metabolism, liver function changes.2discussion purslane (Portulaca oleraeea L.PL) effect and the mechanism of prevention of NAFLD rat model,and provide experimental basis for prevention of nonalcoholic fatty liver disease is a traditional Chinese medicine, but also for the development of purslanepreparation in clinical application and to provide an effective theoretical basis.Method65healthy male SD rats, using randomly divided into model group with55rats and10rats of normal control group, model group rats were daily fed high fat diet, and normal control group of rats given basic diet, normal control group and model group of animal were drinking tap water, the rats in the model group with high fat diet3weeks after feeding and55rats were randomly divided into model control group with15rats and purslane water decoction, in low, high dose group and rosiglitazone group,10rats in each group. After eighth, tenth, Twelfth weeks, weekly random selection model control group of2rats were killed, the liver were stained with H.E, to determine the model control group of rats to severe fatty liver after the end of the experiment, In the course of the experiment carefully observe the general conditions of rats (activity, diet, two), the content of alanine aminotransferase, aspartate aminotransferase, automatic biochemical analyzer detected serum total cholesterol, triglyceride, enzyme linked immunosorbent assay (ELISA) determination of serum leptin, adiponectinResults1weight:compared with the normal control group, model control group and low dose group of decoction of purslane weight increased significantly (P<0.01), but no difference between other group weight; compared with the model group, and no difference in purslane Decoction low dose group of body weight (P>0.05), low dose group, high, weight is higher than that of rosiglitazone group dose group (P<0.01); high dose group, low dose group body weight decreased significantly (P<0.01); no difference in purslane, high dose group and rosiglitazone dose group (P>0.05)2the liver weight:compared with the normal control group, the other group of rats liver weight was significantly increased (P<0.01); compared with the model group, the other group of rats liver weight decreased significantly (P<0.01), model group, low dose significantly increased liver weight (P<0.01), low dose group, middle dose group liver weight than Ming increased significantly (P<0.01); high dose group, middle dose group liver weight was increased (P<0.05); purslane high dose group and the rosiglitazone group had no significant difference (P>0.05).3blood lipids:compared with the normal control group, model group, control group, low dose group TG significantly increased (P<0.01), middle dose group (P<0.05); compared with the model group, low dose group, no difference in TG, the other group were significantly decreased (P<0.01); in middle dose group, low dose of TG reduced differences (P<0.05), the remaining group than in dose of TG significantly decreased (P<0.01); normal group, purslane water decoction, high dose group, rosiglitazone had no difference (P>0.05). Compared with normal control group, the other group TC significantly increased (P<0.01), compared with the model group, and no difference in purslane Decoction low dose group TC (P>0.05), the rest of group TC decreased significantly (P<0.01); no difference in purslane Decoction high dose group, rosiglitazone TC (P>0.05).4liver function:compared with normal control group, model group, Portulaca oleracea Decoction low dose group ALT, AST was significantly increased (P<0.01); compared with the model group, the other group was significantly decreased (P<0.01); low dose group than in the dose group, normal group, high dose group, rosiglitazone group L Gao Xianzhuo (P<0.01) in the higher dose; dose group increased significantly (P<0.01), and no difference in normal group and high dose group, rosiglitazone (P>0.05)5serum leptin:compared with normal control group, rosiglitazone group elevated leptin (P<0.05), model group, Portulaca oleracea Decoction middle dose group and low-dose group leptin significantly increased (P,0.01), Portulaca oleracea Decoction high dose had no difference (P>0.05); compared with the model group, the rest groups were significantly decreased (P<0.01); compared with Portulaca oleracea Decoction low dose group, middle dose reduced difference (P<0.05), normal group and high dose group were significantly decreased (P<0.01); no difference of Portulaca oleracea Decoction high dose group, rosiglitazone group (P>0.05)6adiponectin:compared with normal control group, the other group of adiponectin decreased significantly (P<0.01); the control group compared with the model group, the other group of adiponectin was significantly increased (P<0.01); no difference of Portulaca oleracea Decoction low dose, medium dose group (P>0.05), Portulaca oleracea Decoction low dose group, high dose significantly reduced (P <0.01), and no difference in Portulaca oleracea Decoction high dose group, rosiglitazone (P>0.05).Conclusions1high dose purslane water decoction can effectivelyreduce the non alcoholic fatty liver disease rats serum TG,TC content, AST activity, reduce the ALT, canprevent the formation of NAFLD rat model, speculated that this may be the mechanism of the preventive effects of the NAFLD one.2purslane water decoction, possibly by increasingadiponectin in rat serum leptin content reduction, prevention of non alcoholic fatty liver of rats.3purslane water decoction can obviously improve hepatic pathological changes in NAFLD rats, with inhibition of fatty change and protection of liver function... |
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