Experimental Research On The Funtion Of Anti-Influenza Virus In Vitro Of Yinhuang Qingfei Capsules Containing Serum

Objective:To detection the action on influenza virus A in vitro of Yinhuang Qingfei Capsules containing serum and Preliminary Explore its mechanism of action.Methods:MDCK cells were cultured in vtro with the technology of culture.The dog kidney cells (MDCK cells) were infected by influenza virus A.Yinhuiang Qingfei Capsules containing serum effect on inhibiting Influenza Virus was studied by Chinese medicine pharmacology serological methods and counting cells with MTT assay. Measured in three different dosing ways(Dosing prevention/Therapeutic Use/Direct inhibitory effect).Results:(1) Compare with the corresponding concentration of blank serum, The OD value of Influenza A virus-infected MDCK cells of the concentrations of Yinhuang Qingfei Capsules containing serum and ribavirin tablets containing serum were significantly higher in the three different dosing ways (P<0.05).Compare with the corresponding concentration of ribavirin tablets containing serum, The OD value of Influenza A virus-infected MDCK cells of the concentrations of Yinhuang Qingfei Capsules containing serum have no significantly difference in three different dosing ways CP)0.05)(2) Compared with the model control group, Yinhuang Qingfei Capsules containing serum group cause neuraminidase activity fall to45%,52%,58%,64%,62%,59%,55%,55%.Conclusion:Yinhuang Qingfei Capsules containing serum inhibit influenza virus replication in cells to a certain degree in the three different dosing ways.The mechanism may be related to the inhibition of neuraminidase activity and prevent virus proliferation.