Effects Of The Main Active Components Combinations Between Astragalus And Panax Notoginseng On Inflammatory Reaction After Reperfusion Of Cerebral Ischemia In Mice

Aim To probe the possible mechanisms of the main active components combinations between Astragalus and Panax notoginseng antagonizing on ischemia-reperfusion (I/R) injury from the perspective of the inflammatory response.Methods C57BL/6mice were divided randomly into:sham, model, Astragaloside IV (ASTIV), Ginsenoside Rgl (Rgl), Ginsenoside Rbl (Rbl), Notoginsenoside R1(R1), four active components combination, AST IV+Rg1, ASTIV+Rbl, ASTIV+R1and Edaravone, pretreated for3d. After1h of the last administration, the model of cerebral I/R injury was established by bilateral common carotid artery (CCA) ligation for20min followed by reperfusion for24h. The expression of TNF-a, IL-1β, ICAM-1mRNA in brain tissues were detected by RT-PCR, the expression of p-IκBα as well as NF-κB proteins of cytoplasm and nucleus in brain tissues were tested by western-blot, then, NF-κB nuclear translocation rate was caculated.Results①After cerebral I/R, the expression of TNF-α, IL-1β,ICAM-1mRNA in brain tissues were increased. ASTIV remarkably decreased the expression of TNF-α mRNA,Rg1significantly down-regulated ICAM-1mRNA expression, R1could decrease both TNF-a and ICAM-1mRNA expressions, ASTIV combined with Rg1, Rb1,1Rl had different degree suppression on TNF-α, IL-1β, ICAM-1mRNA, moreover, the inhibitory effects of four active components combination and ASTFV+R1on the inflammatory cytokines were more obvious.②fter cerebral I/R, the expression of p-IκBα protein in brain tissues was notablely increased, NF-κB protein was significantly down-regulated in cytoplasm while up-regulated in nucleus, nuclear translocation rate was raised. ASTIV, Rg1, R1and the active components combinations could prevent the phosphorylation of IκB, relieve the translocation of NF-κB nuclear, furthermore, the effects of the combinations were greater than those of the active components alone. The effects of four active components combination were better than those of ASTIV+Rbl and ASTIV+Rgl.Conclusion The main active components combinations between Astragalus and Panax notoginseng can decrease the productions of the inflammatory cytokines by restraining the activation of NF-κB signaling pathway, thus relieving secondary inflammatory reaction after cerebral ischemia, contributing to the protective effects on brain tissues.