Effect Of Two Novel Inhibitors Of The Biological Activity Of The Tumor Cells In Vitro

Common and frequently occurring cancer is a serious threat to human life and health, occupy the first place in China’s urban and rural mortality, drug treatment is one of the main methods for the treatment of cancer. Global anticancer drugs market sales growth for three consecutive years ranked first, nearly50years of research and development work of anticancer drugs to the tumor chemotherapy have made great progress, but a serious threat to human Life and health accounted for the malignancy than90%of solid tumors has not yet reached a satisfactory effect, there is still half of the cancer patients not responding to treatment or strong resistance and eventually Lead to treatment failure. Therefore, development of new anticancer drugs pharmaceutical companies have to face a very difficult mission.In the1990s, the efficacy of paclitaxel in the treatment of Lung cancer, breast cancer, ovarian cancer and other convenient significantly, oncology and therefore took a step forward, but the paclitaxel formulations of this oil is from ethanol and semi-synthetic castor oil mixture, easy cause allergic reactions in the human body. In recent years, researchers have been Looking for different ways to try to solve this problem, and of yew analogs yew peptide inhibitor chemical structure has been improved and optimized to inhibit cell angiogenesis interfere with the tumor cells in mitosis and targeting tumor treatment. LLL-12is a new type of cells signal transduction molecule as a target for inhibitors of anti-tumor, the targeted drug delivery mechanism of action can make the drug gradually directional at the target site so that the drug concentrated in the Lesion site to play a role in while to achieve the therapeutic purposes of efficiency and Low toxicity.In this study, the use of cell proliferation experiments, DAPI staining, cell invasion assay, flow cytometry analysis, selecting Lung cancer (A549cell line), Liver (HepG-2cell line), uterine cancer (HeLa cells) as a model, research LLL-12inhibitors, cisplatin, the yew peptide inhibitor of cell proliferation inhibition and changes in cell morphology, cell cycle apoptosis and explore a role molecular mechanism of new anti-tumor inhibitors the yew peptides and LLL-12. Specific work are as follows:1The MTT assay yew peptide inhibitor and cisplatin biological activity of cellular carcinoma cells (HepG-2). The results showed that prolonged incubation time with the increase in the concentration of the two compounds, the inhibition rate was gradually increased, and having a time-dose-dependent manner; taxane of different concentrations of the peptide inhibitors of Liver cancer cells (HepG-2) morphology, The results showed the control group more complete cell morphology, membrane damage of the experimental group, the number of cells to reduce or even cell debris; by FCM with PI staining and FITC-PI double staining assay for quantification at different times, different concentrations of yew peptide inhibitors cycle and apoptosis of Liver cancer cells (HepG-2), the results of taxad peptide inhibitors with the drug group cisplatin compared to the same concentrations inhibit the apoptosis of Live cancer (HepG-2) cell.2.The new anti-tumor targeting of inhibitors LLL-12influence on the growth of A549cells, first through the growth curve determine the variation of the growth of A549cells, respectively, of the series of concentrations LLL-12-inhibitor and cisplatin in Lung cancer cells in vitro at different times trypan blue vital stain determination and MTT value-added testing, results show that the more obvious the inhibitor LLL-12the inhibitory activity of the in vitro Lung cancer A549cells; use inverted fluorescence microscopy DAPI staining of a certain concentration of LLL-12the A549cells apoptosis form, the results show that the cells chromatin condensation, cell volume became smaller and smaller, chromosome degradation, nuclear rupture vacuole-Like structure, there are some cell debris observed under a fluorescence microscope, you can see the emergence of apoptotic bodies; cell migration assay with prolonged incubation time72h, compared with the control group, the scratches spacing between the cells of the drug group, join the inducer LLL-12cisplatin treatment chamber can see the rounded atrophy of apoptotic cells, part of the hole room floating cell debris, compared with the control group, a Low concentration of LLL-12role in promoting tumor cell migration rate; FITC-PI double staining quantitative determination of LLL-12inhibitor of Lung Cancer A549impact on the rate of apoptosis analysis showed that the same time, the Low concentration LLL-12cisplatin effect on the cell difference is more obvious, and cisplatin compared LLL-12, inhibitory effect more obvious.