Effects Of Basic Fibroblast Growth Factor On Human Umbilical Cord Mesenchymal Stem Cells Proliferation And Multilineage Differentiation Potential In Vitro

Objective:To observe the effect of basic fibroblast growth factor (bFGF) on humanumbilical cord mesenchymal stem cells (hUC-MSCs) proliferation andmultilineage differentiation potential in vitro.Methods:The adherent culture method. After hUC-MSCs of culture, analysis of itssurface is relatively specific markers by flow cytometry (CD90, CD105, CD73etc.), confirmed that the mesenchymal stem cells; cultured and induced P2generation hUC-MSCs into fat, bone and differentiation into cartilage, in orderto further identification the mesenchymal stem cells, which have the potentialof multi-directional differentiation. Determine bFGF proliferation: divided intoexperimental group and control group, experimental group added bFGF inDMED/F12culture medium, the control group using conventional DMED/F12medium; and the control effect of adding different concentrations of bFGF onthe proliferation of hUC-MSCs was determined by hUC-MSCs; theproliferation ability of MTT method to determine bFGF proliferation, theoptimum concentration was30g/L.Results:The cultured cells was examined by flow cytometry. CD90, CD105, CD73and CD166expression is positive, but not expression of CD34, CD45and HLA-DR, indicating the mesenchymal stem cell phenotype. Oil red O stainingand alizarin red staining were positive, indicating to adipogenic, osteogenicdifferentiation ability. Analysis of growth curve indicated that bFGF couldpromote the proliferation of hUC-MSCs by MTT (the optimum concentrationof30g/L). The7day trend visible cell proliferation culture of continuousdrawing growth curve of hUC-MSCs visible after the induction of hUC-MSCs,from the second day after inoculation with active proliferation of cells in theexperimental group, third days to reach the peak of proliferation, the first4-5days each cell proliferation in plateau.Conclusion:HUC-MSCs has good potential of multi-directional differentiation, bFGFcan promote the proliferation of hUC-MSCs and enhance the differentiation ofhUC-MSCs, lay the foundation and provide plenty of excellent hUC-MSCs forthe subsequent block experiment.