The Study Of Effects And Mechanism Of Curcumin On MM.1R

Objective:To investigate the effects of curcumin on cell growth and apoptosis of multiple myeloma cell line MM.1R in vitro, and to explore the possible mechanism.Methods:The effects of different concentrations of curcumin treatment MM.1R for24,48and72hours were detected by MTT. The apoptosis rate was detected using Annexin V/PI of flow cytometry. Western Blotting was used to test the levels of fragments Caspase-3,8,9, apoptosis-related protein of Bax, apoptosis-restrain protein of XIAP, CIAP and related proteins of signaling pathways MAPK, ERK, AKT and NF-κB. The drug-resistance protein of galectin-3, IL-6, Hsp27were also examined by Western blotting.Results:1. With concentrations respectively of0,10,20,40ug/ml of curcumin treatment MM.1R for24,48and72hours, the results show:tracting on MM.1R after24,48and 72hours by curcumin (10ug/ml), the activity of cells can be reduced, and correlate time; curcumin (20,40ug/ml) can decrease the activity of MM.1R cells greatly contrast to normal controls when it act on MM.1R after24,48and72hours(p<0.05). And its inhibition effect increases with the dose and time.2. With Annexin V/PI flow cytometry detection on concentrations respectively of0,10,20,40ug/ml of curcumin on MM.1R cells after24hours, early apoptosis rates were2.35±1.44%,3.06±2.18%,10.16±3.47%,16.72±4.74%, Curcumin in concentrations of20,40ug/ml can significantly induce apoptosis of MM.1R cells compared with the control group (0ug/ml)(p<0.05), and its effect in inducing apoptosis increases with the concentrations.3. Detection the expression of antiapoptotic proteins and apoptosis protein of MM.1R by Western Blotting, with the concentrations respectively of10,20,40ug/ml of curcumin on MM.1R cells after24hours, the results found that the expression of Caspase-3,Caspase-8,Caspase-9and Bax increased with the concentrations of curcurnin,and the expression of XIAP decline with the increase of concentrations of curcumin contrast to control group(0ug/ml).4. Using Western Blotting detected the protein of signaling pathways of MM.1R, the results found that with the concentrations respectively of10,20,40ug/ml of curcumin on MM.1R cells function after24hours, the expression of signaling protein pMAPKp38, pERKp42/44, pAKT and pNF-κBp65were markedly reduced compared with the control group (0ug/ml).5. Using Western Blotting to detect with concentrations respectively of10,20,40ug/ml of curcumin on MM.1R cells after24hours, results showed that the expression of drug-resistant related proteins of IL-6, galectin-3, and Hsp27were significantly lower contrast to control group(0ug/ml).Conclusions:1. Curcumin can inhibit the proliferation of MM.1R cells, and induce its apoptosis.2. The mechanism of action of curcumin may be inhibiting the activity of MAPK, ERK, AKT and NF-κB pathways and inducing apoptosis rely on Caspase.3. Curcumin have a reversal of drug resistance for MM.1R cells.