Protective Effect Of Boschniakia Rossica Ethanol Extract On Acetaminophen-Induced Acute Liver Injury In Mice

Objective:To study the protective effect of Boschniakia rossica ethanol extract (BREE) on acetaminophen-induced acute the mice liver injury.Methods:1. A mouse model with acute liver injury was induced by intraperitoneally given acetaminophen 300 mg/kg. The levels of serum alanine amino trans ferase (ALT) and aspartate a mino trans fers a e (AST) were measured with the colorimetric methods, and the hepatic histopathologic changes were evaluated under a light microscope at 0,3,6,12 and 24 hour, the best time on mice model of acetaminophen-induced acute liver was identified.2. Kunming mice were used and randomlu divided into the control, model, BREE 100, BREE 200 goups and positive drug reduced silymarin 100 mg/kg group. The control group and silymarin group fed with 20mg/kg saline daily, A mouse model with acute liver injury was induced by intraperitoneally given acetaminophen 300 mg/kg after 1 hour oral administration for 7 days, fasting but water for 16 hours to obtain blood and liver. The levels of ALT and AST, hepatic glutathione and malondialdehyde (MDA) were measured with the colorimetric methods, and the hepatic his to pathologic changes were evaluated under a light microscope. To use protein immunobltting detect the protein expression of cytochrome P450 2E1 (CYP2E1).Results:1.The one-time after inducing acetaminophen 300 mg/kg, the best time zone which acute liver injury in mice was the 12 and 24 hour.2. Compared with control, the level of serum ALT and AST was improved(P< 0.05), the liver has obvious damage, the content of MDA was obviously increased (P< 0.05) and the content of reduced glutathione(GSH) lower (P< 0.05) in model group. Compared with model, in BREE group,the level of serum ALT and AST was decreased significantly(P < 0.05), and the severity of liver injury was improved. The content of MDA was obviously lower(P< 0.05)and the content of reduced glutathione(GSH) increased greatly(P< 0.05). Western blot showed that compared with control, the expression level of CYP2E1 increased in model, compared with model, protein expression level of CYP2E1 decreased in medication group.Conclusion: BREE had a significant protective effect on acetaminophen-induced acute liver injury, and the mechanism may be associated with increment of hepatic GSH and inhibited the protein expression of CYP2E1.