Effect Of Vinegar In Variation Of 16SrDNA On Intestional Flora And Elated Cytokin Expression Level In Mice

Objective:To explore the effect of variation of 16SsDNA expression level of vinegar on 5 strains of bacteria and the related cytokines in change in mice, to explore the vinegar protection of intestinal function and the resistence of inflammatory bowel disease.Methods:24 male C57BL/6 mice were divided into 3 groups randomly.8 mice were fed with vinegar daily as vinegar group, while 16 mice drank water. After 28 days, 8 still drank water as control group, the rest as model group freely drank 3% DSS to induce IBD, the vinegar group fed 3% DSS instead of vinegar. The feces of each mouse were collected and fecal DNA was isolated on day 0,7,14,21 and 28. The total RNA was extracted from mice colon and the RT-PCR carried out immediately. Real-time quantitative assay was performed to detect 16SrDNA expression level of the bifidobacterium, lactobacillus, enterococcus, clostridium and desulfovibrio in the feces to analyze expression differences of IL-10, IL-17A, IL-17F and IL-23.Results:The level of 16SrDNA genes in 5 strains of bacteria in mice feces was analyzed. Compare with the control, the 16SrDNA expression level of lactobacillus was the highest in the experimental group on the day 28 treatment. Bifidobacterium 16SrDNA expression in experimental group varied at different time points,16SrDNA level of bifidobacterium and lactobacillus at high level ranged from 92.61% to 99.09%. However, the amount of both enterococcusand and clostridium 16SrDNA expression level was obviously reduced in the experimental on the 28th. Enterococcus, clostridium and desulfovibrio three kinds of aggressive 16SrDNA levels were significantly low, which only accounted for 0.91%-7.32% in 5 detected bacteria strains in feces.4 kinds of cytokine expression level in 3 groups were compared. The IL-10 expression level in vinegar group was higher than that in model group but lower than that in control group, but no statistical difference was found. The IL-17A secretion in DSS group was slightly higher than that in the vinegar, but the expression in the two groups were significantly lower than that in control group. The comparison of IL-17A level among the 3 groups was not significant. The secretion differences were statistically significant. IL-17F expression in model group was about 5 times higher compared with that in vinegar group (P<0.01). In the control group, the relative expression of IL-23 was decreased, but no obvious statistical significance was found compared with that in both model and vinegar groups.Conclusion:The vinegar has certain positive regulation of intestinal probiotics and inhibitory effect on harmful bacteria. The impacts of vinegar varied on the different cytokines, which plays a protective role in intestinal against inflammatory bowel disease.