| Part1Objectives:To investigate the relationship between serum adipokines and body fat content and distribution in type 2 diabetes.Methods:A total of 40 T2DM patients treated in the First Pepole’s Hostipal of Yunnan Province from July 2013 to November 2013 were enrolled. The height, weight, body mass index (BMI) were measured; serum adipokines were detected by enzyme linked immunosorbent assay (Ray Biotech). Body fat content and lean mass was measured by dual energy X-ray (DEXA). Based on BMI, the subjects were divided into 2 groups:the normal weight group (BMI 18.5-23.9 kg/m2, NW group, n=14) and the over weight/obese group (BMI≥24 kg/m2, OB group, n=26). Two-tailed independent samples t test was used for comparison of normally distributed measurement data between groups. Spearman correlation coefficient was used for correlation analysis.Results:1. Compared with NW group, the level of DPP-4 in OB group was increased significantly ((765.43+72.32ng/mL vs 706.38+91.09ng/mL, t= 2.2480, P= 0.030), while there were no significant difference between the two groups in the level of leptin ((13.3+6.8) ng/ml VS (10.4+6.9) and ADPN (ng/ml) level (7.8+5.3) ng/ml VS (8.1.5.2) ng/ml).2. The level of DPP-4 was positively correlated with body fat content (FAT)(r=0.451, P= 0.003), trunk fat content (TRUNK F) (0.567 P<,0.005) and limb fat content (LIMB F) (r=0.421, P= 0.006). There was no significant correlation between the level of DPP-4 and lean body mass (r=0.180 P=0.265).3.The level of Leptin was positively correlated with body fat content (FAT) (r=0.422, P= 0.006) and limb fat content (LIMB, F) (r=0.502, P= 0.001). There was no significant correlation between the level of Leptin with trunk fat content (TRUNK F) (r=0.230, P= 0.154).4.There was no significant correlation between the level of ADPN with body fat content (FAT) (r=0.0953, P=0.558), trunk fat content (TRUNK F) (r=-0.0482, P=0.768) and limb fat content (LIMB F) (r=0.152, P=0.349).Conclusion:1.The level of DPP-4 of overweight/obese T2DM patients was significantly higher than that of normal weight T2DM patients;2.The level of DPP-4 has a positive correlation with body fat content (FAT), trunk fat content (TRUNKF) and limb fat content (LIMBF),while has no correlation with lean tissue content.3.The level of Leptin has a positive correlation with body fat content (FAT) and limb fat content (LIMBF), while has no correlation with trunk fat content (TRUNKF).4.The level of ADPN has no correlation with body fat content (FAT), trunk fat content (TRUNKF)and limb fat content (LIMBF).Part 2Investigation of serum adipokines with metabolic syndrome and its components in type 2 diabetesObjectives:To investigate the relationship between serum adipokines and metabolic syndrome and its components in type 2 diabetes.Method:A total of 40 T2DM patients treated in the First Pepole’s Hostipal of Yunnan Province from July 2013 to April 2014 were enrolled. The height, weight, body mass index (BMI), blood pressure (BP) were measured; Serum FPG, HbalC, TC, TG, HDL-C, LDL-C and UA were measured by Electrochemiluminescence Method; serum DPP-4, ADPN and leptin were detected by ELISA kit; metabolic syndrome was diagnosed by 2005 IDF criterion.Results:1. Compared with Non-MS group, BMI、DBP、FPG、TC、TG、UA of MS group were significantly higher.2. The relationship between serum DPP4 with metabolic syndrome and its components(1) Compared with Non-MS group, the level of serum DPP-4 was significantly increased in MS group.(2) Compared with control group, the level of serum DPP4 was significantly increased in central obesity group, high TG hyperlipidemia group, hypertension group and female high UA group separately.3. The relationship between serum leptin with metabolic syndrome and its components(1)Compared with Non-MS group, the level of serum leptin was significantly increased in MS group.(2)Compared with control group, the level of serum leptin was significantly increased in central obesity group, high TG hyperlipidemia group, hypertension group and female high UA group separately.4. The relationship between serum adiponectin with metabolic syndrome and its components.(1)Compared with Non-MSgroup, the level of serum ADPN was significantly decreased in MS group.(2)Compared with control group, the level of serum ADPN was significantly decreased in central obesity group, high TG hyperlipidemia group, hypertension group and female high UA group separately.Conclusion:Serum DPP-4, leptin and ADPN may be correlated with metabolic syndrome and its components. Intervention strategies based on adipokines may have potential for future pharmacological treatment of MS.Part 3Effects of DPP4 inhibitor on mRNA expression of inflammatory cytokines and adipokines in adipocytesObjectives:To investigate effecs of DPP4 inhibitor on mRNA expression of inflammatory cytokines [tumor necrosis factor alpha (TNF- alpha), interleukin -6 (IL-6), transforming growth factor beta 3 (TGF- beta 3)] and adipokines [dipeptidyl peptidase-4 (DPP4), leptin (leptin), adiponectin (adiponectin)] in mouse fatty 3T3-L1 cells.Methods:Mouse 3T3-L1 cells were cultured and induced for differentiation. Differentiated 3T3-L1 cells were used for the experiment. Cells were treatedas following:1. DMSO control group; 2, LPS group; cells was stimulated with LPS for 6 hours; 3, Linagliptin +LPS group:cells were firstly pretreated with linagliptin for 1 hour, then stimulated with LPS for 6 hours. The total RNA of each group were extracted and mRNAexpression of inflammatory cytokins and adipokines were detected by qRT-PCR.Results:1. Compared with the control group, mRNA expression of ADPN was significantly decreased in LPS group (P< 0.01); mRNA expression of IL-6 was significantly increased in LPS group (P< 0.05).2. Compared with LPS group, mRNA expression of IL-6 and TNF-a were significantly decreased in linagliptin +LPS group (P< 0.05).Conclusion:DPP-4 inhibitor linagliptin can reduce inflammatory cytokines’ mRNA expression stimulated by LPS and may be related to the inflammatory regulation in fat cells. |
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