AD Cell Model’s Protein Expression Of COX-2/p-GSK-3β And Liuweidihuangwan Serum Intervention Mechanism Research

Objective:By intervening AD cell modle with Aβ25-35 of different concentrations, examine the change of protein expression of cyclooxygenases-2(COX-2),phospho-glycogen synthase kinase 3β(p-GSK-3β)and explore the molecular mechanism of AD. Methods:1 By stimulating C6 cell with Aβ25-35 of different concentrations,observe the cell pr- oliferating activity by useing MTT assay,detect the change of protein expression of COX-2,p-GSK-3β by useing western blot method,selecting the appropriate drug con- centrations to establish AD cell model.2 By stimulating C6 cell with LWDHS of different concentrations,observe the cell proliferating activity by useing MTT assay,detect the change of protein expression of COX-2,p-GSK-3β by western blot,providing basis for the further study of the effect of LWDHS on AD cell model.3 Building AD cell model by use of appropriate concentration Aβ25-35,and useing the appropriate concentration of LWDHS to interve AD cell model, observe the change of protein expression of COX-2,p-GSK-3β by western blot,and explore its molecular mechanism. Results:1 Aβ25-35 intervention C6 cells significantly decreased cell viability in a certain range of concentrations in a dose-dependent manner, the higher the concentration Aβ25-35,inhibition of cell activity is stronger.Western Blot Detection C6 cells expressing COX2, p-GSK-3β results showed that:The expression of COX-2 concentration increased with increasing Aβ25-35,p-GSK-3β protein concentration increased with decr- eased Aβ25-35。2 LWDHS intervention C6 cells,cell activity increased significantly within a certain range of concentrations in a dose-dependent manner,the higher the concentration LWDHS role in promoting cell activity is stronger.Western Blot detection of COX2, the expression of p-GSK-3β results showed that:The expression of COX-2 with LWDHS concentration increased and decreased the expression of p-GSK-3β LWDHS concentration varies with temperature increased.3 Western Blot detection of the control group,the control group, model group, LWDHS COX-2 expression in the intervention group, p-GSK-3β results showed that: LWDHS expression of COX-2 in the intervention group compared with the control group decreased compared with the control group increased,compared with the model group reduced;p-GSK-3β expression of LWDHS intervention group than the control group increased;lower than in the control group compared with the model group. Conclusion:1 Aβ25-35 can significantly inhibit the proliferation of C6 cells in a dose-dependent manner,that the higher the concentration,the more severe the inhibition of cell activity;Aβ25-35 C6 cells were able to stimulate the expression of COX-2 inhibition phosphorylation of GSK-3β inactivation process.2 LWDHS can significantly promote the proliferation of C6 cells, the higher the concentration LWDHS,the stronger the effect of promoting cell activity; LWDHS can downregulate the expression of COX-2 in C6 cells, promoting GSK-3β phosphorylation inactivation.3 LWDHS AD cell mode can improve the situation of AD cell modein the expression of COX-2 down-regulation and improve GSK-3β phosphorylation inactivation inhibition.4 In summary,Aβ25-35 inhibitory activity of C6 cells may increase the expression of COX-2 related,Aβ25-35 for GSK-3β phosphorylation pathway inactivation of COX-2 inhibition may be upregulated reasons;LWDHS possible by promoting the phosphorylation of GSK-3β inactivation pathway,reduced the expression of COX-2,to achieve the protective effect on nerve cells.