The Effect Of The Pioglitazone On The Human Umbilical Vein Endothelial Cell Proliferation And Apoptosis Induced By Advanced Glycation End Produact

Objective:To investigate the effect of Pioglitazone on the human unmilical vein endothelial cell proliferation and apoptosis induced by advanced glycation end product.Method:1) Cultured HUVECs in vitro at passage 2 were used for experiment.2) There were four groups:1.control group; 2.AGEs group:HUVECs were cultured by AGEs with 200ug/ml for 24 hours; 3.Pioglitazone group;HUVECs were cultured by Pioglitazone with 10nmol/ml for 24 hours; 4.AGEs and Pioglitazone group;HUVECs were pre-cultured with 10nmol/ml Pioglitazone for 24 hours,then charged by AGEs with 200ug/ml for 24 hours.Proliferation of HUVECs culcutred for 48 hours were measured with a Cell Counting Kit-8. Absorbance of formazan product,directly proportional to the number of living cells in culture,was measured at 450nm by using a microplate reader. At least 2000 cells were analysed per sample.The effect of AGEs and other drugs on HUVECs apoptosis were investigate with the Annexin V-fluorescein isothiocyanate(FITC) Apoptosis Detection Kit.Results:1) To compared with control, AGEs can inhibit HUVECs proliferation.Compared with AGEs group,the number of HUVECs precultured with PIO,however, is increased.2) To compared with control,AGEs promote HUVECs apoptosis.Compare with AGEs group,the number of HUVECs precultured with PIo is incresed.3) Expression in HUVECs of A20 was increased by AGEs,and the AGEs-induced effect was significantly attenuated by pretreatment with Pioglitazone.Conclusion:The PPAR γ agonist Pioglitazone increased HUVECs function and attenuated HUVECs dysfunction induced by AGEs via inhibiting the NF-kB signal pathway of HUVECs.